Theriault N Y, Carter J B, Pulaski S P
Biopolymer Chemistry, Upjohn Company, Kalamazoo, MI 49001.
Biotechniques. 1988 May;6(5):470-4.
Several phosphorylation, annealing and ligation reaction conditions have been investigated for the enzymatic assembly of genes of interest. The use of longer oligodeoxyribonucleotides (40-60 bases long) also improved the enzymatic reaction. Furthermore, the use of longer oligonucleotides and the elimination of long stretches of G's or C's lowered the mutation rate.
已经对几种磷酸化、退火和连接反应条件进行了研究，以用于感兴趣基因的酶促组装。使用更长的寡脱氧核糖核苷酸（40 - 60个碱基长）也改善了酶促反应。此外，使用更长的寡核苷酸以及消除长片段的鸟嘌呤或胞嘧啶降低了突变率。
