Li D, Fournier M J
Department of Biochemistry and Molecular Biology, Lederle Graduate Research Center, University of Massachusetts, Amherst 01003.
EMBO J. 1992 Feb;11(2):683-9. doi: 10.1002/j.1460-2075.1992.tb05100.x.
The functional equivalency of yeast and mouse U14 RNAs was examined in Saccharomyces cerevisiae. The test RNAs included mouse U14 and several yeast-mouse bi- and tri-partite hybrid RNAs, all transcribed from yeast U14 gene signals. The ability of the heterologous RNAs to provide essential U14 function was assessed in a test strain containing a single glucose-repressible wild-type U14 gene. Mouse U14 was not functional in yeast. However, wild-type growth was supported by hybrid RNAs that included universal sequence elements from either source, two yeast-specific segments and a 5',3' terminal stem domain. The universal sequences include box C, box D and a sequence complementary to 18S rRNA, all shown previously to be required for function of yeast U14. Deletion and substitution mapping defined the yeast-specific elements and showed that a major portion of neighboring non-conserved RNA is dispensible. The results are discussed with a view to defining a minimal consensus U14 molecule.
在酿酒酵母中检测了酵母和小鼠U14 RNA的功能等效性。测试RNA包括小鼠U14以及几种酵母 - 小鼠二元和三元杂交RNA，所有这些均从酵母U14基因信号转录而来。在含有单个葡萄糖可抑制的野生型U14基因的测试菌株中评估异源RNA提供必需U14功能的能力。小鼠U14在酵母中无功能。然而，包含来自任一来源的通用序列元件、两个酵母特异性片段以及5'、3'末端茎结构域的杂交RNA支持野生型生长。通用序列包括框C、框D以及与18S rRNA互补的序列，所有这些先前已表明是酵母U14功能所必需的。缺失和取代图谱确定了酵母特异性元件，并表明相邻非保守RNA的大部分是可有可无的。讨论了这些结果，以期定义一个最小的共有U14分子。
