Department of Nutrition and Dietetics, Faculty of Health Science, Mardin Artuklu University, Mardin, Turkey.
Prep Biochem Biotechnol. 2021;51(2):127-136. doi: 10.1080/10826068.2020.1799390. Epub 2020 Jul 31.
This study aimed to synthesize gold nanoparticles (AuNPs) by hot water extract in room conditions using edible (ME) and investigate the bioactive properties of the synthesized -based gold nanoparticles (ME-AuNPs). The characterization of the biologically synthesized ME-AuNPs was made using the ultraviolet-visible spectrophotometry, X-ray crystallography, scanning electron microscopy, Fourier transforms infrared spectroscopy, and energy dispersive X-ray spectrum methods. The ME-AuNPs, with a particle size of 16.51 nm, were found to have strong bioactive properties. The antioxidant activity of the ME-AuNPs attempted by metal chelating activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity and the β-carotene linoleate model system. The activities at 10 mg/mL were 82, 85, and 77% for the chelation of ferrous ions, DPPH scavenging, and β-carotene linoleate tests, respectively. The ME-AuNPs also showed strong antimicrobial activity against various pathogen microorganisms and strong cytotoxic activity in the A549 and HepG2 cell lines. This study demonstrated the possibility of using a cheap and nontoxic fungal extract as a reducing and stabilizing agent for the synthesis of size-controlled, large-scale, and biocompatible AuNPs that could be used in future diagnostic and therapeutic applications.
本研究旨在通过在室温下使用可食用的（ME）热水提取物合成金纳米粒子（AuNPs），并研究合成的基于的金纳米粒子（ME-AuNPs）的生物活性特性。通过紫外-可见分光光度法、X 射线晶体学、扫描电子显微镜、傅里叶变换红外光谱和能量色散 X 射线光谱法对生物合成的 ME-AuNPs 进行了表征。发现 ME-AuNPs 的粒径为 16.51nm，具有很强的生物活性。通过金属螯合活性、2,2-二苯基-1-苦基肼（DPPH）自由基清除能力和β-胡萝卜素亚油酸模型系统尝试了 ME-AuNPs 的抗氧化活性。在 10mg/mL 时，对亚铁离子螯合、DPPH 清除和β-胡萝卜素亚油酸测试的活性分别为 82%、85%和 77%。ME-AuNPs 还对各种病原体微生物表现出强烈的抗菌活性和在 A549 和 HepG2 细胞系中的强细胞毒性活性。本研究表明，使用廉价且无毒的真菌提取物作为还原和稳定剂来合成尺寸可控、大规模和生物相容的 AuNPs 是可行的，这些 AuNPs 可用于未来的诊断和治疗应用。
