Biosystem and Bioengineering Program, University of Science and Technology (UST), Daejeon, 34141, Republic of Korea.
Plant System Engineering Research Center, Korean Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
Arch Virol. 2023 Apr 16;168(5):141. doi: 10.1007/s00705-023-05734-5.
A novel cytorhabdovirus was identified in Daphne odora in South Korea using high-throughput sequencing. The virus, tentatively named "daphne virus 1" (DV1), has a full-length genome sequence of 13,206 nucleotides with a genome organization comparable to that of unsegmented plant rhabdoviruses and contains seven antisense putative genes in the order 3'-leader-N-P'-P-P3-M-G-L-5'-trailer. The coding region of the genome is flanked by a 3' leader and a 5' trailer sequence, 261 and 151 nucleotides long, respectively. The DV1 genome shares 33.74%-57.44% nucleotide sequence identity with other cytorhabdoviruses. The DV1-encoded proteins share the highest amino acid sequence identity with homologues from Asclepias syriaca virus 1. Phylogenetic analysis showed that DV1 clustered with representative cytorhabdoviruses. We propose classifying DV1 in a new species within the genus Cytorhabdovirus, family Rhabdoviridae.
一种新型细胞弹状病毒在韩国的瑞香中通过高通量测序被鉴定出来。该病毒暂命名为“瑞香病毒 1”（DV1），其全长基因组序列为 13206 个核苷酸，基因组组织与非分段植物弹状病毒相似，包含 7 个反义推定基因，顺序为 3'-leader-N-P'-P-P3-M-G-L-5'-trailer。基因组的编码区被 3'leader 和 5'trailer 序列分别侧翼，长度分别为 261 和 151 个核苷酸。DV1 基因组与其他细胞弹状病毒的核苷酸序列同源性为 33.74%-57.44%。DV1 编码的蛋白与长春花病毒 1 的同源物具有最高的氨基酸序列同一性。系统发育分析表明，DV1 与代表性细胞弹状病毒聚类在一起。我们建议将 DV1 分类为弹状病毒科细胞弹状病毒属的一个新种。
