Department of Restorative Dentistry and Dental Biomaterials, School of Dentistry, University of San Carlos of Guatemala, Guatemala city, Guatemala.
Graduate Program in Dentistry, Federal University of Pelotas, Pelotas, Brazil.
Eur J Oral Sci. 2020 Oct;128(5):436-443. doi: 10.1111/eos.12723. Epub 2020 Aug 2.
In this study, the cytotoxicity of different combinations of contemporary resin-based restoratives (adhesives, composites, luting agents) against human keratinocytes (HaCaT) was evaluated under two conditions, whether materials were applied to dentin or not. Adhesives (3-step etch-and-rinse/3ER: OptiBond FL; 2-step self-etch/2SE Clearfil SE Bond; Single Bond Universal/UNI), composites (conventional composite resin/CCR: Filtek Z350XT; flowable/FCR: Filtek Z350XT Flow; self-adhesive composite resin/SACR: Dyad Flow), and luting agents (conventional luting agent/CLA: Variolink-II; self-adhesive luting agent/SLA: RelyXU200) were combined according to their clinical use. Eluates from polymerized specimens applied to dentin were placed in contact with cells grown for 1 and 7 d. The controls were defined by cells without material contact. Cell viability was determined using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)] assay. C=C conversion was investigated using Fourier-transform infrared spectroscopy. After 1 d of incubation, when dentin was not present, 2SE yielded the highest cell viability, whereas 3ER, UNI, and SACR showed higher cell viability in the presence of dentin. After 7 d, when dentin was absent, 2SE and CLA achieved significantly higher cell viability. The presence of dentin resulted in a drastically higher cell viability for all materials, except 2SE and CLA. UNI had the lowest C=C conversion. The presence of dentin was a significant factor, which resulted in higher cell viability than what was seen for the material specimens per se. All materials resulted in a lower viability of HaCaT than what was seen under the no-material control conditions, with effects mainly limited to the first 24 h.
在这项研究中，评估了在两种情况下（材料是否应用于牙本质）不同当代树脂基修复材料（粘结剂、复合材料、水门汀）组合对人角质细胞（HaCaT）的细胞毒性。粘结剂（三步酸蚀/3ER：OptiBond FL；两步自酸蚀/2SE：Clearfil SE Bond；通用单糊剂/UNI）、复合材料（传统复合树脂/CCR：Filtek Z350XT；流动型/FCR：Filtek Z350XT Flow；自粘接复合树脂/SACR：Dyad Flow）和水门汀（传统水门汀/CLA：Variolink-II；自粘接水门汀/SLA：RelyXU200）根据临床用途进行组合。将聚合后的样本置于牙本质上的浸提液与培养 1 天和 7 天的细胞接触。对照组定义为无材料接触的细胞。使用 MTT [3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴盐]测定细胞活力。使用傅里叶变换红外光谱法研究 C=C 转化率。在不存在牙本质的情况下孵育 1 天后，2SE 表现出最高的细胞活力，而 3ER、UNI 和 SACR 在存在牙本质的情况下显示出更高的细胞活力。7 天后，当牙本质不存在时，2SE 和 CLA 达到了显著更高的细胞活力。除 2SE 和 CLA 外，牙本质的存在导致所有材料的细胞活力显著提高。UNI 的 C=C 转化率最低。牙本质的存在是一个重要因素，导致细胞活力高于材料本身的情况。与无材料对照条件下相比，所有材料都导致 HaCaT 的存活率降低，其影响主要限于前 24 小时。
