Yang Juan, Wang Guowei, Li Haining, Zheng Wenli, Guo Burui, Wang Zhenhai
Department of Neurology, The General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
Ningxia Key Laboratory of Cerebrocranial Diseases, Incubation Base of National Key Laboratory, Yinchuan, Ningxia 750004, P.R. China.
Exp Ther Med. 2020 Aug;20(2):926-932. doi: 10.3892/etm.2020.8745. Epub 2020 May 13.
The ability to inhibit host macrophage apoptosis is one of the survival strategies of intracellular bacteria, including . In the present study the role of MgMn dependent protein phosphatase 1A (PPM1A) in the apoptosis of () strain 2 vaccine-infected BV2 cells was investigated. Compared with control cells, the protein expression levels of cleaved caspase-3 were markedly increased in PPM1A short hairpin (sh)RNA-transfected BV2 cells. Flow cytometry analysis showed that treatment with JNK activator anisomycin significantly increased the rate of apoptosis in BV2 cells in comparison with the control cells. Furthermore, PPM1A shRNA significantly increased the levels of JNK phosphorylation and the levels of cleaved caspase-3 in BV2 cells infected with strain 2 in comparison with the control cells. DAPI staining showed nuclear condensation in infected BV2 cells transfected with PPM1A shRNA in comparison with the control shRNA cells. Flow cytometry analysis showed that PPM1A shRNA significantly increased the percentage of apoptotic BV2 cells infected with strain 2 compared with those transfected with control shRNA. Taken together, these data suggested that knockdown of PPM1A promotes apoptosis in strain 2-infected BV2 cells and that PPM1A may be a potential target in the development of treatments to inhibit intracellular growth of
抑制宿主巨噬细胞凋亡的能力是包括……在内的细胞内细菌的生存策略之一。在本研究中，研究了镁锰依赖性蛋白磷酸酶1A（PPM1A）在感染（）菌株2疫苗的BV2细胞凋亡中的作用。与对照细胞相比，在转染了PPM1A短发夹（sh）RNA的BV2细胞中，裂解的半胱天冬酶-3的蛋白表达水平显著增加。流式细胞术分析表明，与对照细胞相比，用JNK激活剂茴香霉素处理显著增加了BV2细胞的凋亡率。此外，与对照细胞相比，PPM1A shRNA显著增加了感染菌株2的BV2细胞中JNK磷酸化水平和裂解的半胱天冬酶-3水平。DAPI染色显示，与对照shRNA细胞相比，转染了PPM1A shRNA的感染菌株2的BV2细胞中出现核浓缩。流式细胞术分析表明，与转染对照shRNA的细胞相比，PPM1A shRNA显著增加了感染菌株2的凋亡BV2细胞的百分比。综上所述，这些数据表明，敲低PPM1A可促进感染菌株2的BV2细胞凋亡，并且PPM1A可能是开发抑制细胞内生长治疗方法的潜在靶点。
