Pelerito Ana, Cordeiro Rita, Matos Rita, Santos Maria Augusta, Soeiro Sofia, Santos João, Manita Carla, Rio Carla, Santo M, Paixão Eleonora, Nunes Alexandra, Núncio Sofia
Emergency Response and Biopreparedness Unit, Department of Infectious Diseases, National Institute of Health, Lisbon, Portugal.
Immunology Laboratory, Department of Infectious Diseases, National Institute of Health, Lisbon, Portugal.
PLoS One. 2017 Jul 10;12(7):e0179667. doi: 10.1371/journal.pone.0179667. eCollection 2017.
Brucellosis is a zoonosis that is emerging in some regions of the world. Although brucellosis is a disease of obligatory declaration and is not eradicated in Portugal, no prevalence data is available in this country. In this study, we retrospectively analyzed the data available at the Reference Laboratory at the Portuguese National Institute of Health during the past 7 years (2009-2016) in order to get insight into the epidemiological scenario of brucellosis in Portugal. A total of 2313 biological samples from patients with clinical suspicion of brucellosis were subjected to immunological techniques for laboratory diagnosis. From 2010 to 2015, a subset of 259 samples was subjected to molecular methods. According to the available data, 167 out of 2313 (7.2%) samples had positive serology for Brucella spp. and 43 out of 259 samples (16.6%) were positive for B. melitensis by real time PCR, being classified as biovar 1 and 3. This study draws attention to the importance of integrating clinical and laboratory data of human cases in order to increase the efficacy of the response measures in case of outbreaks.
布鲁氏菌病是一种人畜共患病，正在世界一些地区出现。尽管布鲁氏菌病是一种法定报告疾病，在葡萄牙尚未根除，但该国没有患病率数据。在本研究中，我们回顾性分析了葡萄牙国家卫生研究所参考实验室在过去7年（2009 - 2016年）的可用数据，以便深入了解葡萄牙布鲁氏菌病的流行病学情况。共有2313份临床怀疑患有布鲁氏菌病患者的生物样本接受了免疫学技术进行实验室诊断。从2010年到2015年，对259份样本的一个子集进行了分子方法检测。根据现有数据，2313份样本中有167份（7.2%）布鲁氏菌属血清学检测呈阳性，259份样本中有43份（16.6%）通过实时PCR检测出羊种布鲁氏菌呈阳性，被分类为生物变种1和3。本研究提请注意整合人类病例临床和实验室数据对于提高疫情应对措施有效性的重要性。
