College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Int J Biol Macromol. 2020 Nov 15;163:1361-1368. doi: 10.1016/j.ijbiomac.2020.07.247. Epub 2020 Jul 31.
To promote the application of probiotics that are beneficial to hosts, calcium-alginate (Ca-Alg) coated whey protein isolate microcapsules were prepared for protection and delivery of L. bulgaricus and L. paracasei. The internal layer was formed with transglutaminase-induced gelation of whey protein isolate (WPI). Sodium alginate (SA) was applied to form outer layer with external Ca gelation method. The microcapsules loaded with probiotics were characterized by scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FT-IR), and differential scanning calorimetry (DSC). The results showed that the co-encapsulation efficiency was 90.54% and 84.46% of WPI micro-beads and Ca-Alg-coated microcapsules, respectively. The trehalose was added as cryoprotectant to improve the survival rate of probiotics in freeze-dried Ca-Alg-coated microcapsules from 3% to 41.26%. Ca-Alg-coated microcapsules have regular morphology and intensified structure. The protection of Ca-Alg-coated microcapsule for probiotics was improved under simulated gastrointestinal and thermal conditions. Ca-Alg-WPI microcapsules showed a useful way for protection and delivery of L. bulgaricus and L. paracasei.
为了促进有益宿主的益生菌的应用，用海藻酸钠（SA）包埋乳清分离蛋白微胶囊来保护和输送保加利亚乳杆菌和副干酪乳杆菌。内层通过转谷氨酰胺酶诱导乳清分离蛋白（WPI）的胶凝形成。采用外部 Ca 凝胶化方法将 SA 应用于形成外层。用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）和差示扫描量热法（DSC）对负载益生菌的微胶囊进行了表征。结果表明，WPI 微球和 Ca-Alg 包被微胶囊的包埋效率分别为 90.54%和 84.46%。添加海藻糖作为冷冻保护剂可将益生菌在 Ca-Alg 包被微胶囊中的冷冻干燥存活率从 3%提高到 41.26%。Ca-Alg 包被微胶囊具有规则的形态和强化的结构。在模拟胃肠道和热条件下，Ca-Alg 包被微胶囊对益生菌的保护作用得到了提高。Ca-Alg-WPI 微胶囊为保加利亚乳杆菌和副干酪乳杆菌的保护和输送提供了一种有用的方法。
