School of Food Science and Biotechnology, Zhejiang Gongshang University, Xiasha, Hangzhou, Zhejiang 310018, PR China.
School of Food Science and Biotechnology, Zhejiang Gongshang University, Xiasha, Hangzhou, Zhejiang 310018, PR China; School of Science, Faculty of Engineering and Science, University of Greenwich, Central Avenue, Chatham ME4 4TB, UK.
Int J Biol Macromol. 2023 Jul 1;242(Pt 4):125030. doi: 10.1016/j.ijbiomac.2023.125030. Epub 2023 May 25.
To improve the viability of Lacticaseibacillus rhamnosus ZFM231 strain in the gastrointestinal tract and exhibit better probiotic effect, an internal emulsification/gelation technique was employed to encapsulate this strain using whey protein and pectin as wall materials to fabricate the double layer microcapsules. Four key factors affecting the encapsulation process were optimized using single factor analysis and response surface methodology. Encapsulation efficiency of L. rhamnosus ZFM231 reached 89.46 ± 0.82 %, the microcapsules possessed a particle size of 172 ± 1.80 μm and ζ-potential of -18.36 mV. The characters of the microcapsules were assessed using optical microscope, SEM, FT-IR and XRD analysis. It was found that after exposure to simulated gastric fluid, the bacterial count (log (CFU g)) of the microcapsules only lost 1.96 units, the bacteria were released readily in simulated intestinal fluid, reaching 86.56 % after 90 min. After stored at 4 °C for 28 days and 25 °C for 14 days, bacterial count of the dry microcapsules decreased from 10.59 to 9.02 and 10.49 to 8.70 log (CFU g), respectively. The double layered microcapsules could significantly increase the storage and thermal abilities of bacteria. Such L. rhamnosus ZFM231 microcapsules could find applications as ingredient of the functional foods and the dairy products.
为提高鼠李糖乳杆菌 ZFM231 菌株在胃肠道中的存活率并展现更好的益生菌效果，采用内乳化/凝胶技术，以乳清蛋白和果胶为壁材，制备双层微胶囊包埋该菌株。采用单因素分析和响应面法优化了影响包埋过程的四个关键因素。鼠李糖乳杆菌 ZFM231 的包埋效率达到 89.46 ± 0.82%，微胶囊粒径为 172 ± 1.80 μm，ζ-电位为-18.36 mV。采用光学显微镜、SEM、FT-IR 和 XRD 分析对微胶囊的特性进行了评估。结果发现，在模拟胃液中暴露后，微胶囊中的细菌计数（log（CFU g））仅损失 1.96 个单位，在模拟肠液中细菌很容易释放，90 min 后达到 86.56%。在 4℃储存 28 天和 25℃储存 14 天后，干燥微胶囊中的细菌计数分别从 10.59 降至 9.02 和从 10.49 降至 8.70 log（CFU g）。双层微胶囊可以显著提高细菌的储存和耐热能力。这种鼠李糖乳杆菌 ZFM231 微胶囊可以作为功能性食品和乳制品的成分得到应用。
