Department of Stomatology, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.
Department of Plastic Surgery, Shengjing Hospital affiliated to China Medical University, Shenyang, Liaoning, China.
Stem Cell Res Ther. 2020 Aug 3;11(1):338. doi: 10.1186/s13287-020-01858-6.
The repair of critical-sized bone defect represents a challenging problem in bone tissue engineering. To address the most important problem in bone defect repair, namely insufficient blood supply, this study aimed to find a method that can promote the formation of vascularized bone tissue.
The phenotypes of ASCs and EPCs were identified respectively, and ASCs/EPCs were co-cultured in vitro to detect the expression of osteogenic and angiogenic genes. Furthermore, the co-culture system combined with scaffold material was used to repair the critical-sized bone defects of the cranial bone in rats.
The co-culture of ASCs/EPCs could increase osteogenesis and angiogenesis-related gene expression in vitro. The results of in vivo animal experiments demonstrated that the ASC/EPC group could promote bone regeneration and vascularization in the meantime and then significantly accelerate the repair of critical-sized bone defects.
It is feasible to replace traditional single seed cells with ASC/EPC co-culture system for vascularized bone regeneration. This system could ultimately enable clinicians to better repair the defect of craniofacial bone and avoid donor site morbidity.
临界尺寸骨缺损的修复是骨组织工程中的一个挑战性问题。为了解决骨缺损修复中最重要的问题，即血供不足，本研究旨在寻找一种能够促进血管化骨组织形成的方法。
分别鉴定 ASC 和 EPC 的表型，并在体外共培养 ASC/EPC 以检测成骨和成血管基因的表达。此外，还使用共培养系统结合支架材料修复大鼠颅骨临界尺寸骨缺损。
ASC/EPC 的共培养可增加体外成骨和成血管相关基因的表达。体内动物实验结果表明，ASC/EPC 组可同时促进骨再生和血管生成，从而显著加速临界尺寸骨缺损的修复。
用 ASC/EPC 共培养系统替代传统的单一种子细胞进行血管化骨再生是可行的。该系统最终可以使临床医生更好地修复颅面骨的缺损，避免供体部位的发病率。
