从白及中提取、纯化胃保护活性葡甘聚糖及其测定。
Extraction, purification, and determination of the gastroprotective activity of glucomannan from Bletilla striata.
机构信息
National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, 710119, PR China.
Shaanxi Pharmaceutical Holding Group PAIANG Chinese Medicine Co., Xi'an, 110026, PR China.
出版信息
Carbohydr Polym. 2020 Oct 15;246:116620. doi: 10.1016/j.carbpol.2020.116620. Epub 2020 Jun 12.
In this study, a water-soluble polysaccharide (BSP) was extracted and purified from pseudobulb of Bletilla striata. The preliminary structure and gastroprotective activity of BSP were analyzed. Results indicate that BSP is a glucomannan with a molar ratio of 7.45:2.55 (Man:Glc), and its molecular weight is approximately 1.7 × 10 Da. BSP displayed outstanding protective action against ethanol-induced GES-1 cell injury in vitro, as well as, excellent gastroprotective activity in vivo. Especially, a high-dose of BSP (100 mg/kg) could reduce the ulcer index of the gastric mucosa and increase the percentage of ulcer inhibition, which possibly caused by enhancing the antioxidant capacity and inhibiting the apoptotic pathway in gastric tissue. Interestingly, BSP exhibited a comparative gastroprotective activity to that of positive control (omeprazole). In summary, our results indicated that BSP could be considered as a potential supplement for the prevention of gastric injury.
在这项研究中，从白芨假鳞茎中提取并纯化了一种水溶性多糖（BSP）。分析了 BSP 的初步结构和胃保护活性。结果表明，BSP 是一种甘露糖：葡萄糖摩尔比为 7.45:2.55（Man:Glc）的葡甘露聚糖，分子量约为 1.7×10 Da。BSP 对乙醇诱导的 GES-1 细胞损伤具有显著的体外保护作用，并且在体内也具有优异的胃保护活性。特别是，高剂量的 BSP（100mg/kg）可以降低胃黏膜的溃疡指数并增加溃疡抑制率，这可能是通过增强抗氧化能力和抑制胃组织中的凋亡途径来实现的。有趣的是，BSP 表现出与阳性对照（奥美拉唑）相当的胃保护活性。总之，我们的结果表明，BSP 可以被认为是预防胃损伤的潜在补充剂。
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