Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu 210037, China.
SUST Think Tank for Urban Development, Suzhou University of Science and Technology, Suzhou, Jiangsu 215009, China.
Phytopathology. 2021 Feb;111(2):293-303. doi: 10.1094/PHYTO-05-20-0170-R. Epub 2021 Jan 13.
Anthracnose caused by is one of most serious fungal diseases on Chinese fir (). Eight fungal endophytes were isolated from a young heathy branch of Chinese fir and screened against the pathogen in vitro. One isolate, designated as SMEL1 and subsequently identified as based on morphological and phylogenetic analyses, suppressed mycelial growth of on dual-culture plates. Additionally, metabolites significantly decreased the biomass of . was able to enter the internal tissues of the host plant via stomatal cells. Metabolites of significantly inhibited conidial germination and appressorium formation, which at least partly explained why the endophyte significantly inhibited lesion development caused by on various host plants. We further confirmed that some components with antifungal activity could be extracted from using ethyl acetate as an organic solvent. To our knowledge, this is the first report of as a potential biocontrol agent against a fungal phytopathogen.
由 引起的炭疽病是中国冷杉（）上最严重的真菌病害之一。从健康的中国冷杉幼枝上分离到 8 株内生真菌，并在体外对病原菌进行了筛选。其中一个分离株，命名为 SMEL1，根据形态学和系统发育分析，被鉴定为 ，它在双培养平板上抑制了 的菌丝生长。此外， 代谢物显著降低了 的生物量。 能够通过气孔细胞进入宿主植物的内部组织。 的代谢物显著抑制了分生孢子的萌发和附着胞的形成，这至少部分解释了为什么内生真菌能显著抑制 对各种宿主植物造成的损伤发展。我们进一步证实，使用乙酸乙酯作为有机溶剂，可以从 中提取出具有抗真菌活性的成分。据我们所知，这是首次报道 作为一种潜在的生防因子来防治真菌病原体。
