Holkar Somnath K, Ghotgalkar Prabhavati S, Lodha Tushar D, Bhanbhane Vrushali C, Shewale Shraddha A, Markad Harshvardhan, Shabeer A T P, Saha Sujoy
Indian Council of Agricultural Research-National Research Centre for Grapes, Pune, Maharashtra 412307 India.
National Centre of Cell Science, Pune, Maharashtra 411 007 India.
3 Biotech. 2023 Jul;13(7):258. doi: 10.1007/s13205-023-03675-z. Epub 2023 Jul 1.
In the present study, 51 fungal endophytes (FEs) were isolated, purified and identified from the healthy leaf segments of ten grapevine varieties based on the spore and colony morphologies and ITS sequence information. The FEs belonged to the Ascomycota division comprising eight genera viz., , , , , and . The in vitro direct confrontation assay against revealed that six isolates viz., VR8 (70%), SB2 (83.15%), CS2 (88.42%), MN3 (88.42%), MS5 (78.94%) and MS15 (78.94%) inhibited the mycelial growth of test pathogen. The remaining 45 fungal isolates showed 20-59.9% growth inhibition of Indirect confrontation assay manifested that the isolates MN1 and MN4a showed 79.09% and 78.18% growth inhibition of followed by MM4 (73.63%) and S5 (71.81%) isolates. Isolate S5 and MM4 were found to produce azulene and 1,3-Cyclopentanedione, 4,4-dimethyl as antimicrobial volatile organic compounds, respectively. The 38 FEs showed PCR amplification using internal transcribed spacer universal primers. The BLAST search revealed highest similarity with the existing sequences in the database. The phylogenetic analysis revealed the occurrence of seven distinct clusters each corresponding to single genus.
The online version contains supplementary material available at 10.1007/s13205-023-03675-z.
在本研究中，基于孢子和菌落形态以及ITS序列信息，从十个葡萄品种的健康叶片片段中分离、纯化并鉴定了51种真菌内生菌（FE）。这些FE属于子囊菌门，包括八个属，即 ， ， ， ， ， ， 和 。针对 的体外直接对峙试验表明，六种分离株，即VR8（70%）、SB2（83.15%）、CS2（88.42%）、MN3（88.42%）、MS5（78.94%）和MS15（78.94%）抑制了测试病原菌的菌丝生长。其余45种真菌分离株对 的生长抑制率为20 - 59.9%。间接对峙试验表明，分离株MN1和MN4a对 的生长抑制率分别为79.09%和78.18%，其次是MM4（73.63%）和S5（71.81%）分离株。发现分离株S5和MM4分别产生薁和4,4 - 二甲基 - 1,3 - 环戊二酮作为抗菌挥发性有机化合物。38种FE使用内部转录间隔区通用引物进行了PCR扩增。BLAST搜索显示与数据库中现有序列的相似度最高。系统发育分析揭示了七个不同的簇，每个簇对应一个属。
在线版本包含可在10.1007/s13205 - 023 - 03675 - z获取的补充材料。
