Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin, China.
Guizhou University of Traditional Chinese Medicine, Guiyang, China.
World J Microbiol Biotechnol. 2022 Oct 25;38(12):242. doi: 10.1007/s11274-022-03434-x.
Saikosaponin d (SSd) is an important bioactive compound of traditional Chinese medicinal plant Bupleurum scorzonerifolium Willd. and exhibits many effects, such as anti-tumor, anti-inflammation and immunomodulatory. Since endophytic fungi possess the natural capacity to produce the similar secondary metabolite to that of their host plants, they are promising as alternative sources of plant bioactive natural products. In this study, in order to search for SSd-producing strains, endophytes were isolated from B. scorzonerifolium and were authenticated by the ITS sequence and the translation elongation factor-1alpha gene (TEF-1α) sequence analysis. The profile of metabolites present in the crude exacts was carried out by ultra performance liquid chromatography time-of-flight mass spectrometry (UPLC/Q-TOF-MS) analysis. The results showed that two strains, CHS and CHS from B. scorzonerifolium could produce SSd by UPLC/Q-TOF-MS analysis, and the amount of SSd produced by strain CHS and CHS were about 2.17 and 2.40 µg/mL, respectively. CHS and CHS showed a close phylogenetic relationship to Fusarium oxysporum and Fusarium acuminatum, respectively. According to our concern, no endophytic fungi capable of producing SSd from B. scorzonerifolium have been found before. Our clear intention was to isolate and identify these endophytic fungi that produce important active secondary metabolites, and then study the strains that produce this compound on a large scale through fermentation or even genetic study, to provide a feasible and more convenient way for the production of SSd.
柴胡皂苷 d（SSd）是中国传统药用植物柴胡中的一种重要生物活性化合物，具有抗肿瘤、抗炎和免疫调节等多种作用。由于内生真菌具有天然产生与其宿主植物相似的次生代谢产物的能力，因此它们是植物生物活性天然产物的有前途的替代来源。在这项研究中，为了寻找产生 SSd 的菌株，从柴胡中分离出内生真菌，并通过 ITS 序列和翻译延伸因子-1α 基因（TEF-1α）序列分析进行鉴定。通过超高效液相色谱飞行时间质谱（UPLC/Q-TOF-MS）分析对粗提取物中存在的代谢物进行分析。结果表明，通过 UPLC/Q-TOF-MS 分析，来自柴胡的两个菌株 CHS 和 CHS 可以产生 SSd，菌株 CHS 和 CHS 产生的 SSd 量分别约为 2.17 和 2.40μg/mL。CHS 和 CHS 与尖孢镰刀菌和旋孢镰刀菌分别具有密切的系统发育关系。据我们所知，以前从未从柴胡中发现能够产生 SSd 的内生真菌。我们的明确意图是分离和鉴定这些能够产生重要活性次生代谢产物的内生真菌，然后通过发酵甚至遗传研究对产生这种化合物的菌株进行大规模研究，为 SSd 的生产提供一种可行且更方便的方法。
