School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
Alabama Agricultural Experiment Station, Auburn, AL 36849, USA.
Int J Mol Sci. 2020 Aug 2;21(15):5535. doi: 10.3390/ijms21155535.
The transition from fertilized egg to larva in fish is accompanied with various biological processes. We selected seven early developmental stages in channel catfish, , for transcriptome analysis, and covered 22,635 genes with 590 million high-quality RNA-sequencing (seq) reads. Differential expression analysis between neighboring developmental timepoints revealed significantly enriched biological categories associated with growth, development and morphogenesis, which was most evident at 2 vs. 5 days post fertilization (dpf) and 5 vs. 6 dpf. A gene co-expression network was constructed using the Weighted Gene Co-expression Network Analysis (WGCNA) approach and four critical modules were identified. Among candidate hub genes, , , and were involved in head formation, egg development and the transverse central element of synaptonemal complexes. , , and were mainly associated with regulation of cell cycle, growth, brain development, differentiation and proliferation of enterocytes. and were critical for the regulation of immune activity and ion transport. Additionally, and were related to phosphate transport and regulating cell proliferation. All these genes play vital roles in embryogenesis and regulation of early development. These results serve as a rich dataset for functional genomic studies. Our work reveals new insights of the underlying mechanisms in channel catfish early development.
鱼类从受精卵到幼虫的转变伴随着各种生物学过程。我们选择了斑点叉尾鮰的七个早期发育阶段进行转录组分析，共覆盖了 22635 个基因，使用了 5.9 亿条高质量的 RNA 测序（seq）reads。相邻发育时间点之间的差异表达分析揭示了与生长、发育和形态发生相关的显著富集的生物学类别，在受精后 2 天与 5 天和 5 天与 6 天之间最为明显。使用加权基因共表达网络分析（WGCNA）方法构建了一个基因共表达网络，鉴定出了四个关键模块。在候选枢纽基因中， 、 、 和 参与头部形成、卵子发育和联会复合体的横向中央元件。 、 、 和 主要与细胞周期调控、生长、脑发育、肠细胞分化和增殖有关。 和 对免疫活性和离子转运的调节至关重要。此外， 和 与磷酸盐转运和调节细胞增殖有关。所有这些基因在胚胎发生和早期发育的调控中都起着至关重要的作用。这些结果为功能基因组研究提供了丰富的数据集。我们的工作揭示了斑点叉尾鮰早期发育潜在机制的新见解。
