Nóvoa Lourdes, Sánchez María Del Carmen, Blanco Juan, Limeres Jacobo, Cuenca Maigualida, Marín María José, Sanz Mariano, Herrera David, Diz Pedro
Medical-Surgical Dentistry Research Group, Health Research Institute of Santiago de Compostela, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Etiology and Therapy of Periodontal Diseases Research Group, University Complutense of Madrid, 28040 Madrid, Spain.
J Clin Med. 2020 Aug 2;9(8):2482. doi: 10.3390/jcm9082482.
To describe the subgingival microbiome of individuals with Down syndrome (DS).
We conducted a cross-sectional observational study that obtained bacterial DNA samples from 50 patients with DS, 25 with periodontitis (PDS) and 25 with a healthy periodontal condition (HDS). The samples were analyzed by sequencing the 16S rRNA gene V3-V4 hypervariable region using the MiSeq System. Taxonomic affiliations were assigned using the naïve Bayesian classifier integrated in QIIME2 plugins. We evaluated the difference in bacteria abundance between the sample groups using Wilcoxon and Kruskal-Wallis tests. We evaluated the alpha diversity of the identified species using the Observed, Chao1metric, ACE and Shannon indices and evaluated beta diversity with principal coordinate analysis (registration code: 2018/510).
Twenty-one genera and 39 bacterial species showed a significantly different abundance between the study groups. Among the genera, , , and were more abundant in the PDS group than in the HDS group, as were the less commonly studied , and genera. Among the species, spp. and spp. were the most abundant in the PDS group; the most abundant species in the HDS group were spp., spp. and spp.
Well-recognized periodontal pathogens and newly proposed pathogenic taxa were associated with periodontitis in patients with DS.
描述唐氏综合征（DS）患者的龈下微生物群。
我们进行了一项横断面观察性研究，从50例DS患者中获取细菌DNA样本，其中25例患有牙周炎（PDS），25例牙周状况健康（HDS）。使用MiSeq系统对16S rRNA基因V3-V4高变区进行测序来分析样本。使用QIIME2插件中集成的朴素贝叶斯分类器确定分类归属。我们使用Wilcoxon和Kruskal-Wallis检验评估样本组之间细菌丰度的差异。我们使用观察到的、Chao1度量、ACE和香农指数评估已鉴定物种的α多样性，并通过主坐标分析评估β多样性（注册号：2018/510）。
21个属和39种细菌在研究组之间显示出显著不同的丰度。在这些属中， 、 和 在PDS组中比在HDS组中更丰富, 研究较少的 、 和 属也是如此。在这些物种中， spp.和 spp.在PDS组中最为丰富；HDS组中最丰富的物种是 spp.、 spp.和 spp.。
公认的牙周病原体和新提出的致病分类群与DS患者的牙周炎有关。
