Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China.
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China; First Clinical Division, Peking University School and Hospital of Stomatology, Beijing, China.
Arch Oral Biol. 2019 May;101:92-99. doi: 10.1016/j.archoralbio.2019.02.012. Epub 2019 Mar 4.
The aim of the study was to profile the subgingival microbiome of Chinese adults with generalized aggressive periodontitis (GAgP) using human oral microbe identification microarray (HOMIM), and to compare the results with matched periodontal healthy controls.
15 subjects with GAgP and 15 age- and gender- matched periodontal healthy controls were included. Subgingival plaque samples were collected from the deepest pockets of patients with GAgP and matched sites in controls and then analyzed by 16S rRNA-based microarrays. Student's paired t-test was used to compare clinical parameters and mean number of bacterial taxa detected between the two groups. Fisher's exact probability test and Wilcoxon Rank Sum were used to compare bacterial species between all samples. A multiple linear regression model was used for correlations among age, gender and bacterial with clinical parameters.
From a total sum of 379 strains tested, 171 bacterial strains were detected from subgingival plaques of the GAgP patients, more than the 157 strains detected in control group. Mean number of subgingival bacterial taxa detected in GAgP group was 68 (SD = 21.06) while in control group was 45 (SD = 21.60). 47 bacterial taxa were detected more frequently in GAgP group while 12 taxa were more prevalent in control group. The significantly more prevalent and abundant taxa of bacteria in GAgP group included Filifactor alocis, Desulfobulbus sp., Fretibacterium sp., Porphyromonas gingivalis, Tannerella forsythia, Porphyromon as endodontalis, Peptostreptococcaceae spp., Parvimonas micra, Eubacterium nodatum and Eubacterium saphenum. Meanwhile the more abundant taxa in control group were Streptococcus spp. and Pseudomonas aeruginosa.
There are more taxa of bacteria in subgingival plaques of Chinese patients with GAgP than in healthy controls. F. alocis, Desulfobulbus sp., Fretibacterium sp., P. gingivalis and T. forsythia are strongly associated with GAgP. High-throughout microbiological results may help dentists have a better understanding of subgingival microbiome of GAgP.
本研究旨在通过人类口腔微生物鉴定微阵列（HOMIM）对患有广泛性侵袭性牙周炎（GAgP）的中国成年人的龈下微生物组进行分析，并将结果与匹配的牙周健康对照组进行比较。
本研究纳入了 15 名 GAgP 患者和 15 名年龄和性别匹配的牙周健康对照组。从 GAgP 患者的最深牙周袋和对照组的匹配部位采集龈下菌斑样本，然后通过 16S rRNA 微阵列进行分析。采用学生配对 t 检验比较两组之间的临床参数和检测到的细菌分类单元的平均数量。采用 Fisher 确切概率检验和 Wilcoxon 秩和检验比较所有样本中的细菌种类。采用多元线性回归模型分析年龄、性别和细菌与临床参数之间的相关性。
在总共测试的 379 种菌株中，从 GAgP 患者的龈下菌斑中检测到 171 种细菌菌株，多于对照组中检测到的 157 种。GAgP 组龈下细菌分类单元的平均数量为 68（SD=21.06），而对照组为 45（SD=21.60）。在 GAgP 组中检测到 47 种细菌分类单元更为常见，而在对照组中则有 12 种更为常见。GAgP 组中更为常见和丰富的细菌分类单元包括 Filifactor alocis、Desulfobulbus sp.、Fretibacterium sp.、Porphyromonas gingivalis、Tannerella forsythia、Porphyromonas as endodontalis、Peptostreptococcaceae spp.、Parvimonas micra、Eubacterium nodatum 和 Eubacterium saphenum。而在对照组中更为丰富的细菌分类单元则包括 Streptococcus spp. 和 Pseudomonas aeruginosa。
与健康对照组相比，中国 GAgP 患者的龈下菌斑中存在更多的细菌分类单元。F. alocis、Desulfobulbus sp.、Fretibacterium sp.、P. gingivalis 和 T. forsythia 与 GAgP 密切相关。高通量微生物学结果可能有助于牙医更好地了解 GAgP 的龈下微生物组。
