Jin Sun Woo, Lee Gi Ho, Jang Min Jung, Hong Gyeong Eun, Kim Jae Young, Park Gi Deok, Jin Hui, Kim Hyun Su, Choi Jae Ho, Choi Chul Yung, Lee Su Gwon, Jeong Hye Gwang, Hwang Yong Pil
College of Pharmacy, Chungnam National University, Daejeon 34134, Korea.
Department of Research, GREEN CROSS Wellbeing Co., Ltd., Seongnam 13595, Korea.
Microorganisms. 2020 Aug 2;8(8):1175. doi: 10.3390/microorganisms8081175.
Recently, subsp. has been reported to have immunostimulating properties in an immunosuppressed-animal model. However, the immunological activities of and the molecular mechanisms remain unclear. In this report, we evaluated the immunostimulating activity and associated mechanisms of subsp. GCWB1176 (GCWB1176) in macrophages and cyclophosphamide (CTX)-induced immunosuppressed mice. In a series of safety tests, GCWB1176 was found to have a negative response to hemolysis, as well as susceptibility to antibiotics. Administration of GCWB1176 elevated natural killer (NK) cell activities; concanavalin A-induced T cell proliferation; and serum levels of tumor necrosis factor (TNF)-α, interferon (IFN)-γ, interleukin (IL)-2, IL-4, IL-10 and IL-12 in CTX-induced immunosuppressed mice. In RAW264.7 macrophages, treatment with GCWB1176 induced phagocytic activity and increased the production of nitric oxide (NO) and expression of inducible NO synthase. Simultaneously, GCWB1176 increased the production of TNF-α, IFN-γ, IL-1β, IL-10 and IL-12 from mouse splenocytes and RAW264.7 cells. In addition, GCWB1176 significantly increased the transcriptional activities of NF-κB and iNOS. Taken together, GCWB1176 improved immune function through the activation of macrophages and NK cells. These findings suggest that dietary supplementation of GCWB1176 may be used to enhance immunity.
最近，据报道该亚种在免疫抑制动物模型中具有免疫刺激特性。然而，其免疫学活性及分子机制仍不清楚。在本报告中，我们评估了该亚种GCWB1176在巨噬细胞和环磷酰胺（CTX）诱导的免疫抑制小鼠中的免疫刺激活性及相关机制。在一系列安全性测试中，发现GCWB1176对溶血呈阴性反应，且对抗生素敏感。给予GCWB1176可提高CTX诱导的免疫抑制小鼠的自然杀伤（NK）细胞活性、伴刀豆球蛋白A诱导的T细胞增殖，以及肿瘤坏死因子（TNF）-α、干扰素（IFN）-γ、白细胞介素（IL）-2、IL-4、IL-10和IL-12的血清水平。在RAW264.7巨噬细胞中，用GCWB1176处理可诱导吞噬活性，并增加一氧化氮（NO）的产生及诱导型NO合酶的表达。同时，GCWB1176增加了小鼠脾细胞和RAW264.7细胞中TNF-α、IFN-γ、IL-1β、IL-10和IL-12的产生。此外，GCWB1176显著增加了NF-κB和iNOS的转录活性。综上所述，GCWB1176通过激活巨噬细胞和NK细胞改善免疫功能。这些发现表明，膳食补充GCWB1176可用于增强免疫力。
