解析抗体组学：过去、现在和未来。

Dissecting the antibody-OME: past, present, and future.

机构信息

Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA.

出版信息

Curr Opin Immunol. 2020 Aug;65:89-96. doi: 10.1016/j.coi.2020.06.003. Epub 2020 Aug 2.

DOI:10.1016/j.coi.2020.06.003

PMID:32755751

Abstract

Humoral immunity is key to protection for nearly all licensed vaccines. Yet, the design of vaccines has been more difficult for some of our most deadly killers (e.g. HIV, influenza, Dengue virus, etc.), likely due to our incomplete understanding of the precise immunological mechanisms associated with protection. Humoral immunity is governed both by B-cells and their bi-functional secreted antibodies, all of which have a unique capacity to evolve during an immune response. Current OMIC technologies capture individual features of the humoral immune response, providing a glimpse into humoral components (Fab/Fc/B-cell-omic), but fail to provide a wholistic view of the humoral response as a collective functional arm. Here, we dissect current OMIC strategies reviewing experimental and computational approaches, that if integrated could provide a true systems-level view of the humoral immune response.

摘要

体液免疫是几乎所有获得许可的疫苗保护的关键。然而，对于我们一些最致命的杀手（例如 HIV、流感、登革热病毒等），疫苗的设计更加困难，这可能是由于我们对与保护相关的确切免疫机制的理解不完整。体液免疫既受 B 细胞及其双功能分泌抗体的控制，所有这些抗体在免疫反应过程中都具有独特的进化能力。当前的 OMIC 技术可捕获体液免疫反应的个体特征，使我们对体液成分（Fab/Fc/B 细胞组学）有了一定的了解，但未能提供作为一个整体功能臂的体液反应的全貌。在这里，我们通过审查实验和计算方法来剖析当前的 OMIC 策略，如果将这些方法整合在一起，就可以为体液免疫反应提供真正的系统级视图。