快速鉴定当克隆到多拷贝质粒上时具有致死性的细菌基因。
Rapid identification of bacterial genes that are lethal when cloned on multicopy plasmids.
作者信息
Berg C M, Liu L, Wang B, Wang M D
机构信息
Department of Molecular and Cell Biology, University of Connecticut, Storrs 06268.
出版信息
J Bacteriol. 1988 Jan;170(1):468-70. doi: 10.1128/jb.170.1.468-470.1988.
Abstract
A procedure to identify genes that are lethal when cloned on multicopy plasmids was developed. It depends on the ability of mini-Mu plasmid elements to be used for both in vivo cloning and generalized transduction of enterobacterial genes. The feasibility of this procedure was demonstrated by using the tetA gene of Tn10, which is lethal when in multiple copies in the presence of 25 micrograms of tetracycline per ml.
摘要
开发了一种鉴定在多拷贝质粒上克隆时具有致死性的基因的方法。它依赖于小型 Mu 质粒元件用于体内克隆和肠道细菌基因的普遍性转导的能力。通过使用 Tn10 的 tetA 基因证明了该方法的可行性，当每毫升存在 25 微克四环素时，该基因多拷贝存在时具有致死性。
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