• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

微小 Mu 质粒噬菌体介导的高频广义转导

High frequency generalized transduction by miniMu plasmid phage.

作者信息

Wang B M, Liu L, Groisman E A, Casadaban M J, Berg C M

出版信息

Genetics. 1987 Jun;116(2):201-6. doi: 10.1093/genetics/116.2.201.

DOI:10.1093/genetics/116.2.201
PMID:3301525
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1203130/
Abstract

Deletion derivatives of phage Mu which replicate as multicopy plasmids, and also transpose and package like Mu, have been developed for the in vivo cloning of bacterial genes. We show here that these miniMu plasmid phage are also efficient at generalized transduction and that both in vivo cloning and generalized transduction of a given gene can be accomplished in a single experiment.

摘要

已经开发出噬菌体Mu的缺失衍生物,它们作为多拷贝质粒进行复制,并且像Mu一样进行转座和包装,用于细菌基因的体内克隆。我们在此表明,这些miniMu质粒噬菌体在广义转导方面也很高效,并且给定基因的体内克隆和广义转导都可以在单个实验中完成。

相似文献

1
High frequency generalized transduction by miniMu plasmid phage.微小 Mu 质粒噬菌体介导的高频广义转导
Genetics. 1987 Jun;116(2):201-6. doi: 10.1093/genetics/116.2.201.
2
In vivo cloning of DNA into multicopy cosmids by mini-Mu-cosduction.通过mini-Mu转导将DNA在体内克隆到多拷贝黏粒中。
Mol Gen Genet. 1986 Dec;205(3):546-9. doi: 10.1007/BF00338096.
3
In vivo transfer of chromosomal mutations onto multicopy plasmids by transduction with bacteriophage P1.通过噬菌体P1转导将染色体突变体内转移到多拷贝质粒上。
Gene. 1985;40(2-3):241-6. doi: 10.1016/0378-1119(85)90046-0.
4
In vivo DNA cloning and adjacent gene fusing with a mini-Mu-lac bacteriophage containing a plasmid replicon.利用含有质粒复制子的微型Mu - lac噬菌体进行体内DNA克隆及相邻基因融合。
Proc Natl Acad Sci U S A. 1984 Mar;81(5):1480-3. doi: 10.1073/pnas.81.5.1480.
5
Replacement of the fip gene of Escherichia coli by an inactive gene cloned on a plasmid.用克隆在质粒上的无活性基因替换大肠杆菌的fip基因。
J Bacteriol. 1984 Sep;159(3):1034-9. doi: 10.1128/jb.159.3.1034-1039.1984.
6
[Integration of phage Mu into the RP4::D3112A-plasmid in Escherichia coli cells].[噬菌体Mu在大肠杆菌细胞中整合到RP4::D3112A质粒中]
Genetika. 1989 Aug;25(8):1384-90.
7
Generalized transduction between Salmonella typhi and Salmonella typhimurium by phage j2 and characterization of the j2 plasmid in Escherichia coli.噬菌体j2介导的伤寒沙门氏菌和鼠伤寒沙门氏菌之间的广义转导以及大肠杆菌中j2质粒的特性分析
J Gen Microbiol. 1983 Nov;129(11):3395-400. doi: 10.1099/00221287-129-11-3395.
8
Mini-Mu transposition of bacterial genes on the transmissible plasmid.
Folia Microbiol (Praha). 1987;32(5):368-75. doi: 10.1007/BF02887566.
9
Transduction of Myxococcus virescens by coliphage P1CM: generation of plasmids containing both phage and Myxococcus genes.用大肠杆菌噬菌体P1CM转导绿粘球菌:产生含有噬菌体和绿粘球菌基因的质粒。
J Gen Microbiol. 1978 Jul;107(1):73-83. doi: 10.1099/00221287-107-1-73.
10
Lysogenization by satellite phage P4.卫星噬菌体P4的溶原化作用。
Virology. 1981 Aug;113(1):20-38. doi: 10.1016/0042-6822(81)90133-1.

引用本文的文献

1
Protein ProQ influences osmotic activation of compatible solute transporter ProP in Escherichia coli K-12.蛋白质ProQ影响大肠杆菌K-12中相容性溶质转运蛋白ProP的渗透激活。
J Bacteriol. 1999 Mar;181(5):1537-43. doi: 10.1128/JB.181.5.1537-1543.1999.
2
Rapid identification of bacterial genes that are lethal when cloned on multicopy plasmids.快速鉴定当克隆到多拷贝质粒上时具有致死性的细菌基因。
J Bacteriol. 1988 Jan;170(1):468-70. doi: 10.1128/jb.170.1.468-470.1988.
3
Cloning and characterization of the Escherichia coli K-12 alanine-valine transaminase (avtA) gene.大肠杆菌K-12丙氨酸-缬氨酸转氨酶(avtA)基因的克隆与特性分析
J Bacteriol. 1987 Sep;169(9):4228-34. doi: 10.1128/jb.169.9.4228-4234.1987.
4
Cloning of genes that suppress an Escherichia coli K-12 alanine auxotroph when present in multicopy plasmids.对存在于多拷贝质粒中时可抑制大肠杆菌K-12丙氨酸营养缺陷型的基因进行克隆。
J Bacteriol. 1987 Dec;169(12):5610-4. doi: 10.1128/jb.169.12.5610-5614.1987.
5
Tn5tac1, a derivative of transposon Tn5 that generates conditional mutations.Tn5tac1,转座子Tn5的一种衍生物,可产生条件性突变。
Proc Natl Acad Sci U S A. 1988 Sep;85(17):6468-72. doi: 10.1073/pnas.85.17.6468.
6
Rapid sequencing of cloned DNA using a transposon for bidirectional priming: sequence of the Escherichia coli K-12 avtA gene.利用转座子进行双向引物介导的克隆DNA快速测序:大肠杆菌K-12 avtA基因的序列
Nucleic Acids Res. 1987 Nov 25;15(22):9461-9. doi: 10.1093/nar/15.22.9461.
7
In vivo cloning of Pseudomonas aeruginosa genes with mini-D3112 transposable bacteriophage.利用mini-D3112转座噬菌体对铜绿假单胞菌基因进行体内克隆。
J Bacteriol. 1989 Jul;171(7):3917-25. doi: 10.1128/jb.171.7.3917-3925.1989.
8
A functional leuABCD operon is required for leucine synthesis by the tyrosine-repressible transaminase in Escherichia coli K-12.功能性亮氨酸操纵子(leuABCD)是大肠杆菌K-12中酪氨酸可抑制转氨酶合成亮氨酸所必需的。
J Bacteriol. 1991 Jun;173(12):3864-71. doi: 10.1128/jb.173.12.3864-3871.1991.

本文引用的文献

1
Analysis of an avtA::Mu d1(Ap lac) mutant: metabolic role of transaminase C.avtA::Mu d1(Ap lac)突变体分析:转氨酶C的代谢作用
J Bacteriol. 1982 May;150(2):739-46. doi: 10.1128/jb.150.2.739-746.1982.
2
Infection of Salmonella typhimurium with coliphage Mu d1 (Apr lac): construction of pyr::lac gene fusions.用大肠杆菌噬菌体Mu d1(Apr lac)感染鼠伤寒沙门氏菌:构建pyr::lac基因融合体。
J Bacteriol. 1981 Jan;145(1):299-305. doi: 10.1128/jb.145.1.299-305.1981.
3
In vivo DNA cloning and adjacent gene fusing with a mini-Mu-lac bacteriophage containing a plasmid replicon.利用含有质粒复制子的微型Mu - lac噬菌体进行体内DNA克隆及相邻基因融合。
Proc Natl Acad Sci U S A. 1984 Mar;81(5):1480-3. doi: 10.1073/pnas.81.5.1480.
4
Mini-Mu-duction as a test for genetic complementation in Escherichia coli.微量转导作为大肠杆菌基因互补性的一种检测方法。
J Bacteriol. 1984 Apr;158(1):357-61. doi: 10.1128/jb.158.1.357-361.1984.
5
Transposition derivatives of an Hfr strain of Escherichia coli K-12.大肠杆菌K-12 Hfr菌株的转座衍生物
Genetics. 1967 Jul;56(3):503-25. doi: 10.1093/genetics/56.3.503.
6
beta-Chloro-L-alanine inhibition of the Escherichia coli alanine-valine transaminase.β-氯-L-丙氨酸对大肠杆菌丙氨酸-缬氨酸转氨酶的抑制作用。
J Bacteriol. 1985 Dec;164(3):1350-2. doi: 10.1128/jb.164.3.1350-1352.1985.
7
Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria.来自耐氯霉素细菌的氯霉素乙酰转移酶。
Methods Enzymol. 1975;43:737-55. doi: 10.1016/0076-6879(75)43141-x.