微小 Mu 质粒噬菌体介导的高频广义转导
High frequency generalized transduction by miniMu plasmid phage.
作者信息
Wang B M, Liu L, Groisman E A, Casadaban M J, Berg C M
出版信息
Genetics. 1987 Jun;116(2):201-6. doi: 10.1093/genetics/116.2.201.
Abstract
Deletion derivatives of phage Mu which replicate as multicopy plasmids, and also transpose and package like Mu, have been developed for the in vivo cloning of bacterial genes. We show here that these miniMu plasmid phage are also efficient at generalized transduction and that both in vivo cloning and generalized transduction of a given gene can be accomplished in a single experiment.
摘要
已经开发出噬菌体Mu的缺失衍生物，它们作为多拷贝质粒进行复制，并且像Mu一样进行转座和包装，用于细菌基因的体内克隆。我们在此表明，这些miniMu质粒噬菌体在广义转导方面也很高效，并且给定基因的体内克隆和广义转导都可以在单个实验中完成。
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本文引用的文献
1
Analysis of an avtA::Mu d1(Ap lac) mutant: metabolic role of transaminase C.avtA::Mu d1（Ap lac）突变体分析：转氨酶C的代谢作用
J Bacteriol. 1982 May;150(2):739-46. doi: 10.1128/jb.150.2.739-746.1982.
2
Infection of Salmonella typhimurium with coliphage Mu d1 (Apr lac): construction of pyr::lac gene fusions.用大肠杆菌噬菌体Mu d1（Apr lac）感染鼠伤寒沙门氏菌：构建pyr::lac基因融合体。
J Bacteriol. 1981 Jan;145(1):299-305. doi: 10.1128/jb.145.1.299-305.1981.
3
In vivo DNA cloning and adjacent gene fusing with a mini-Mu-lac bacteriophage containing a plasmid replicon.利用含有质粒复制子的微型Mu - lac噬菌体进行体内DNA克隆及相邻基因融合。
Proc Natl Acad Sci U S A. 1984 Mar;81(5):1480-3. doi: 10.1073/pnas.81.5.1480.
4
Mini-Mu-duction as a test for genetic complementation in Escherichia coli.微量转导作为大肠杆菌基因互补性的一种检测方法。
J Bacteriol. 1984 Apr;158(1):357-61. doi: 10.1128/jb.158.1.357-361.1984.
5
Transposition derivatives of an Hfr strain of Escherichia coli K-12.大肠杆菌K-12 Hfr菌株的转座衍生物
Genetics. 1967 Jul;56(3):503-25. doi: 10.1093/genetics/56.3.503.
6
beta-Chloro-L-alanine inhibition of the Escherichia coli alanine-valine transaminase.β-氯-L-丙氨酸对大肠杆菌丙氨酸-缬氨酸转氨酶的抑制作用。
J Bacteriol. 1985 Dec;164(3):1350-2. doi: 10.1128/jb.164.3.1350-1352.1985.
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Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria.来自耐氯霉素细菌的氯霉素乙酰转移酶。
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