Raybaud F, Noguchi T, Marics I, Adelaide J, Planche J, Batoz M, Aubert C, de Lapeyriere O, Birnbaum D
Unité 119 de l'INSERM, Marseille, France.
Cancer Res. 1988 Feb 15;48(4):950-3.
We have used an assay combining DNA-mediated gene transfer and tumorigenicity in Swiss athymic mice to look for activated ras genes in solid human sporadic melanomas. This assay can detect ras oncogenes mutated at codons 12, 13, or 61. We examined a panel of 13 independent surgical specimens of primary tumors and metastases. No H- or K-ras oncogenes were detected; an N-ras oncogene, mutated at codon 61, was identified in one of the 13 samples. No N-ras genes mutated at codon 13 were detected. Thus, the tumorigenicity assay detects a low frequency of ras gene activation in melanomas.
我们采用了一种结合DNA介导的基因转移和瑞士无胸腺小鼠致瘤性的检测方法，来寻找人类散发性实体黑色素瘤中的活化ras基因。该检测方法可检测在密码子12、13或61处发生突变的ras癌基因。我们检测了一组由13个原发性肿瘤和转移灶的独立手术标本。未检测到H-或K-ras癌基因；在13个样本中的1个样本中鉴定出1个在密码子61处发生突变的N-ras癌基因。未检测到在密码子13处发生突变的N-ras基因。因此，致瘤性检测方法检测到黑色素瘤中ras基因活化的频率较低。
