Wiraguna Anak Agung Gde Putra, Hari Embun Dini, Praharsini I Gusti Ayu Agung
Dermatology and Venereology Department, Faculty of Medicine, Universitas Udayana-Sanglah General Hospital, Denpasar, Bali, Indonesia.
Clin Cosmet Investig Dermatol. 2020 Jul 9;13:455-459. doi: 10.2147/CCID.S258834. eCollection 2020.
Melasma is a condition of hyperpigmentation of the facial skin that increases in prevalence with ageing. The alleged involvement of reactive oxygen species and antioxidants is the basis of the pathology of melasma. Glutathione is one of the non-enzymatic antioxidants produced by the body and plays a role in melanogenesis. The purpose of this study was to examine serum glutathione levels on the severity of melasma.
This study used a cross-sectional design conducted at the Cosmetic Dermatology Clinic at Sanglah Hospital, Denpasar, from September to October 2016. Serum glutathione was examined through venous blood with ELISA method, and the severity of melasma was assessed using melasma area severity index (MASI). Independent -test and ANOVA were used to evaluate differences in plasma glutathione levels based on the characteristics of the sample. Pearson correlation test and linear regression were used to assess the relationship between MASI and plasma glutathione.
This study involved 47 people with a clinical diagnosis of melasma. There was a significant strong negative correlation between plasma glutathione and MASI (p<0.001; r = -0.624). Mild melasma (1.89 ± 0.28 µmol/L) had higher plasma glutathione levels compared to moderate melasma (1.53 ± 0.13 µmol/L) and severe (1.18 ± 0.20 µmol/L) (p=0.043). Linear regression showed a significant negative linear relationship between MASI scores against plasma glutathione (β = -58.2; p <0.01).
Glutathione plasma has a strong negative correlation with the MASI score in person with melasma.
黄褐斑是一种面部皮肤色素沉着过度的病症，其患病率随年龄增长而增加。活性氧和抗氧化剂被认为与黄褐斑的病理机制有关。谷胱甘肽是人体产生的非酶抗氧化剂之一，在黑色素生成中起作用。本研究的目的是检测血清谷胱甘肽水平与黄褐斑严重程度之间的关系。
本研究采用横断面设计，于2016年9月至10月在登巴萨桑格拉医院的美容皮肤科诊所进行。通过静脉血采用酶联免疫吸附测定法检测血清谷胱甘肽，并使用黄褐斑面积严重程度指数（MASI）评估黄褐斑的严重程度。采用独立样本t检验和方差分析根据样本特征评估血浆谷胱甘肽水平的差异。采用Pearson相关检验和线性回归分析评估MASI与血浆谷胱甘肽之间的关系。
本研究纳入了47例临床诊断为黄褐斑的患者。血浆谷胱甘肽与MASI之间存在显著的强负相关（p<0.001；r = -0.624）。轻度黄褐斑患者（1.89±0.28µmol/L）的血浆谷胱甘肽水平高于中度（1.53±0.13µmol/L）和重度（1.18±0.20µmol/L）黄褐斑患者（p=0.043）。线性回归显示MASI评分与血浆谷胱甘肽之间存在显著的负线性关系（β = -58.2；p <0.01）。
黄褐斑患者的血浆谷胱甘肽与MASI评分呈强负相关。
