College of Food Science and Engineering, Hainan University, Haikou, China.
Key Laboratory of Food Nutrition and Functional Food in Hainan Province, Hainan University, Haikou, China.
Front Immunol. 2020 Jul 17;11:1542. doi: 10.3389/fimmu.2020.01542. eCollection 2020.
Previous studies of (), which is mainly distributed in tropical areas, have mainly focused on its functional polysaccharide; the effects of polyphenols, the chemical composition of which may be improved by fermentation, have received limited attention, especially in models, which inevitably involve interactions with intestinal microorganisms. To address this challenge, metagenomic and metabolomic techniques, were applied, and immune factors and mucosal barrier-related proteins were determined to reveal the effects of fermented polyphenols (FDC) on intestinal inflammation induced by oxazolone in zebrafish. The results showed that fermentation significantly changed the chemical composition of and that FDC significantly improved the intestinal immune index. After the 21st day of FDC intervention, the abundance of , and increased, but the abundance of the genera , and decreased. At the same time, FDC significantly increased intestinal short-chain fatty acids (SCFAs). In addition, network analysis based on multi-omics indicated that FDC intake leads to changes in intestinal microbiota and intestinal metabolites, resulting in enhanced host immune function. These results indicate that FDC can improve intestinal health by regulating the intestinal microbiota and its metabolites to treat intestinal inflammation and regulate the host immune system. The present research improved our understanding of the utilization of polyphenols and provided new evidence for the impacts of fermented on host health.
先前对 （）的研究主要集中在其功能多糖上，该物质主要分布在热带地区；而其化学组成可能通过发酵得到改善的多酚的影响则受到较少关注，特别是在 模型中，这不可避免地涉及与肠道微生物的相互作用。为了应对这一挑战，应用了宏基因组学和代谢组学技术，测定了免疫因子和黏膜屏障相关蛋白，以揭示发酵 多酚（FDC）对氧化偶氮甲烷诱导的斑马鱼肠道炎症的影响。结果表明，发酵显著改变了 的化学组成，FDC 显著改善了肠道免疫指数。在 FDC 干预的第 21 天， 、 和 的丰度增加，但 、 和 的丰度降低。同时，FDC 显著增加了肠道短链脂肪酸（SCFAs）。此外，基于多组学的网络分析表明，FDC 的摄入导致肠道微生物群和肠道代谢物的变化，从而增强了宿主的免疫功能。这些结果表明，FDC 可以通过调节肠道微生物群及其代谢物来改善肠道健康，从而治疗肠道炎症并调节宿主免疫系统。本研究提高了我们对多酚利用的认识，并为发酵 对宿主健康的影响提供了新的证据。
