George Nadja S, Cheung Lumei, Luthria Devanand L, Santin Monica, Dawson Harry D, Bhagwat Arvind A, Smith Allen D
Environmental Microbial and Food Safety Lab Beltsville Agricultural Research Center Agricultural Research Service, Department of Agriculture Beltsville Maryland.
Diet Genomics and Immunology Lab Beltsville Human Nutrition Research Center Agricultural Research Service, Department of Agriculture Beltsville Maryland.
Food Sci Nutr. 2019 Jul 7;7(8):2565-2576. doi: 10.1002/fsn3.1106. eCollection 2019 Aug.
Treatment of mice with a pomegranate peel extract (PPX) decreased the pathogenicity of () infections. Here, we investigate the effects of PPX on the microbiome of uninfected or -infected C3H/HeNCr mice by 16S rRNA gene sequencing. Mice were treated with water or PPX for 14 days, feces were collected, and then, the mice were infected with and feces collected again at day 6 postinfection. DNA was isolated from the fecal samples and subjected to 16S rRNA gene sequencing to determine the microbial composition. Differences in the composition of the microbiome were observed for untreated and PPX-treated mice with PPX mice having decreased diversity. PPX treatment decreased the Firmicutes/Bacteroidetes ratio by increasing Bacteroidetes and decreasing Firmicutes levels. The decrease in Firmicutes was driven by a large reduction in . PPX treatment increased the abundance of Proteobacteria and Verrucomicrobiae and decreased Actinobacteria. The relative abundance of reached 22% in water-treated but only 5% in PPX-treated infected mice. These results suggest that consumption of pomegranate polyphenols altered the microbiome, making it more resistant to displacement by infection with , indicating that pomegranate polyphenols may mitigate the pathogenic effects of food-borne bacterial pathogens.
用石榴皮提取物（PPX）处理小鼠可降低（）感染的致病性。在此，我们通过16S rRNA基因测序研究PPX对未感染或感染的C3H/HeNCr小鼠微生物群的影响。小鼠用水或PPX处理14天，收集粪便，然后感染，在感染后第6天再次收集粪便。从粪便样本中分离DNA并进行16S rRNA基因测序以确定微生物组成。观察到未处理和PPX处理的小鼠微生物群组成存在差异，PPX处理的小鼠多样性降低。PPX处理通过增加拟杆菌门水平和降低厚壁菌门水平降低了厚壁菌门/拟杆菌门的比例。厚壁菌门的减少是由（）的大幅减少驱动的。PPX处理增加了变形菌门和疣微菌门的丰度，降低了放线菌门的丰度。在水处理的感染小鼠中，（）的相对丰度达到22%，而在PPX处理的感染小鼠中仅为5%。这些结果表明，食用石榴多酚会改变微生物群，使其更能抵抗（）感染的替代，这表明石榴多酚可能减轻食源细菌性病原体的致病作用。
