Xia Ke, Wu Qiaofen, Yang Yanni, Liu Qiao, Wang Zaihua, Zhao Zhiguo, Li Jie, He Jinxiang, Chai Shengfeng, Qiu Shuo
Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences/Guangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization, Guilin 541006, China.
Guangdong Provincial Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Int J Mol Sci. 2024 Feb 27;25(5):2731. doi: 10.3390/ijms25052731.
Many species, which hold a high status and value in traditional Chinese medicine, grow on barks and rocks in the wild, often encountering harsh environments and facing droughts. However, the molecular mechanisms underlying the shift in the photosynthetic pathway induced by drought remain unclear. To address this issue, three species with different photosynthetic pathways were selected for sequencing and transcriptome data analysis after drought treatment. The findings included 134.43 GB of sequencing data, with numerous Differentially Expressed Genes (DEGs) exhibiting different response mechanisms under drought stress. Gene Ontology (GO)-KEGG-based enrichment analysis of DEGs revealed that metabolic pathways contributed to drought tolerance and alterations in photosynthetic pathways. Phosphoenolpyruvate Carboxylase (PEPC) was subjected to phylogenetic tree construction, sequence alignment, and domain analysis. Under drought stress, variations were observed in the PEPC gene structure and expression among different species; the upregulation of expression may be caused by dof-miR-384, which resulted in the shift from C photosynthesis to CAM, thereby improving drought tolerance in . This study revealed the expression patterns and roles of PEPC genes in enhancing plant drought tolerance and will provide an important basis for in-depth research on 's adaptation mechanisms in arid environments.
许多在传统中药中具有很高地位和价值的物种生长在野外的树皮和岩石上，常常遭遇恶劣环境并面临干旱。然而，干旱诱导光合途径转变的分子机制仍不清楚。为解决这一问题，选择了三种具有不同光合途径的物种进行干旱处理后的测序和转录组数据分析。研究结果包括134.43GB的测序数据，大量差异表达基因（DEGs）在干旱胁迫下表现出不同的响应机制。基于基因本体论（GO）-京都基因与基因组百科全书（KEGG）的DEGs富集分析表明，代谢途径有助于耐旱性和光合途径的改变。对磷酸烯醇式丙酮酸羧化酶（PEPC）进行了系统发育树构建、序列比对和结构域分析。在干旱胁迫下，不同物种的PEPC基因结构和表达存在差异；表达上调可能是由dof-miR-384引起的，这导致了从C光合作用向景天酸代谢（CAM）的转变，从而提高了[物种名称未提及]的耐旱性。本研究揭示了PEPC基因在增强植物耐旱性中的表达模式和作用，将为深入研究[物种名称未提及]在干旱环境中的适应机制提供重要依据。
