Cao Yunpeng, Meng Dandan, Li Xiaoxu, Wang Lihu, Cai Yongping, Jiang Lan
Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, China.
Key Lab of Non-wood Forest Products of State Forestry Administration, College of Forestry, Central South University of Forestry and Technology, Changsha, China.
Front Plant Sci. 2020 Jul 15;11:1087. doi: 10.3389/fpls.2020.01087. eCollection 2020.
BZR transcription factors play essential roles in plant growth and environmental stimuli, and they are also the positive regulators of Brassinosteroid (BR) signal transduction in diverse plants. In addition, BZR TFs, as crucial regulators of BR synthesis, may have multiple stress-resistance functions and their related regulatory mechanisms have been well illustrated in model plants. Here, we carried out a genome-wide identification of members in Chinese pear () and identified 13 members. By comparative analysis in five Rosaceae genomes, members in the pear genome may have undergone large-scale duplication events during evolution. Purifying selection played an important role in almost all of the orthologous and paralogous gene pairs. According to the expression analysis of the during fruit development, three were selected for detailed analysis. Transcriptional activation assays presented that repressed the promoters of lignin biosynthetic genes, such as , , and . Our study traces the evolution of gene family members in Rosaceae genomes and illustrates that the rates of gene loss and gain are far from equilibrium in different species. At the same time, our results suggest that may be involved in the negative regulation of lignin biosynthesis.
BZR转录因子在植物生长和环境刺激中发挥着重要作用，并且它们也是多种植物中油菜素内酯（BR）信号转导的正调控因子。此外，BZR转录因子作为BR合成的关键调控因子，可能具有多种抗逆功能，其相关调控机制已在模式植物中得到充分阐明。在此，我们对中国梨（）中的成员进行了全基因组鉴定，共鉴定出13个成员。通过对五个蔷薇科基因组的比较分析，梨基因组中的成员在进化过程中可能经历了大规模的复制事件。纯化选择在几乎所有直系同源和旁系同源基因对中都发挥了重要作用。根据果实发育过程中的表达分析，选择了三个进行详细分析。转录激活试验表明，抑制了木质素生物合成基因如、和的启动子。我们的研究追踪了蔷薇科基因组中基因家族成员的进化，并表明不同物种中基因丢失和获得的速率远非平衡。同时，我们的结果表明可能参与了木质素生物合成的负调控。
