Ye Miao, Zhang Zhengcan, Huang Guanjun, Xiong Zhuang, Peng Shaobing, Li Yong
Ministry of Agriculture Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China.
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, China.
AoB Plants. 2020 Jun 19;12(4):plaa028. doi: 10.1093/aobpla/plaa028. eCollection 2020 Aug.
The intraspecific variations of leaf structure and anatomy in rice leaves and their impacts on gas diffusion are still unknown. Researches about the tradeoff between structural compositions and intracellular chemical components within rice leaves are still lacking. The objectives of the present study were to investigate the varietal differences in leaf structure and leaf chemical compositions, and the tradeoff between leaf structural tissues and intracellular chemical components in rice leaves. Leaf structure, leaf anatomy, leaf chemical composition concentrations and gas exchange parameters were measured on eight L. genotypes to investigate the intraspecific variations in leaf structure and leaf anatomy and their impacts on gas exchange parameters, and to study the tradeoff between leaf structural compositions (cell wall compounds) and intracellular chemical components (non-structural carbohydrates, nitrogen, chlorophyll). Leaf thickness increased with leaf mass per area (LMA), while leaf density did not correlate with LMA. Mesophyll cell surface area exposed to intercellular airspace (IAS) per leaf area, the surface area of chloroplasts exposed to IAS and cell wall thickness increased with LMA. Cell wall compounds accounted for 71.5 % of leaf dry mass, while mass-based nitrogen and chlorophyll concentrations decreased with LMA. Mesophyll conductance was negatively correlated with LMA and cell wall thickness. High LMA rice genotypes invest more leaf mass to cell wall and possess a low mesophyll conductance.
水稻叶片结构和解剖结构的种内变异及其对气体扩散的影响尚不清楚。关于水稻叶片结构组成与细胞内化学成分之间权衡关系的研究仍然缺乏。本研究的目的是调查叶片结构和叶片化学成分的品种差异，以及水稻叶片结构组织与细胞内化学成分之间的权衡关系。对8个水稻基因型测定了叶片结构、叶片解剖结构、叶片化学成分浓度和气体交换参数，以研究叶片结构和叶片解剖结构的种内变异及其对气体交换参数的影响，并研究叶片结构组成（细胞壁化合物）与细胞内化学成分（非结构性碳水化合物、氮、叶绿素）之间的权衡关系。叶片厚度随单位面积叶质量（LMA）增加而增加，而叶片密度与LMA无相关性。单位叶面积暴露于细胞间隙（IAS）的叶肉细胞表面积、暴露于IAS的叶绿体表面积和细胞壁厚度随LMA增加而增加。细胞壁化合物占叶片干质量的71.5%，而基于质量的氮和叶绿素浓度随LMA降低。叶肉导度与LMA和细胞壁厚度呈负相关。高LMA水稻基因型在细胞壁上投入更多的叶质量，且叶肉导度较低。
