Tekedar Hasan C, Arick Mark A, Hsu Chuan-Yu, Thrash Adam, Blom Jochen, Lawrence Mark L, Abdelhamed Hossam
College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, United States.
Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, MS, United States.
Front Cell Infect Microbiol. 2020 Jul 17;10:348. doi: 10.3389/fcimb.2020.00348. eCollection 2020.
is a Gram-negative species ubiquitous in different aquatic environments and capable of causing a variety of diseases to a broad host range. species have the capability to carry and acquire antimicrobial resistance (AMR) elements, and currently multi-drug resistant (MDR) isolates are commonly found across the world. strain MS-17-88 is a MDR strain isolated from catfish in the southeastern United States. The present study was undertaken to uncover the mechanism of resistance in MDR strain MS-17-88 through the detection of genomic features. To achieve this, genomic DNA was extracted, sequenced, and assembled. The strain MS-17-88 genome comprised 5,178,226-bp with 58.6% G+C, and it encoded several AMR elements, including , and . The phylogeny and resistance profile of a large collection of strains, including MS-17-88, were evaluated. Phylogenetic analysis showed a close relationship between MS-17-88 and strain Ae5 isolated from fish in China and ARB3 strain isolated from pond water in Japan, indicating a common ancestor of these strains. Analysis of phage elements revealed 58 intact, 63 incomplete, and 15 questionable phage elements among the 53 genomes. The average phage element number is 2.56 per genome, and strain MS-17-88 is one of two strains having the maximum number of identified prophage elements (6 elements each). The profile of resistance against various antibiotics across the 53 genomes revealed the presence of , and in all genomes (100%). By comparison, and were detected in 7.5% and 1.8% of genomes. Nearly 77% of strains carried , and 7.5% of strains carried . This result suggested a low abundance and prevalence of sulfonamide and florfenicol resistance genes compared with tetracycline resistance among strains. Overall, the present study provides insights into the resistance patterns among 53 genomes, which can inform therapeutic options for fish affected by .
是一种革兰氏阴性菌，在不同的水生环境中普遍存在，能够对广泛的宿主范围引发多种疾病。该菌种有携带和获取抗菌抗性（AMR）元件的能力，目前多重耐药（MDR）菌株在世界各地普遍存在。MS-17-88菌株是从美国东南部鲶鱼中分离出的多重耐药菌株。本研究旨在通过检测基因组特征来揭示多重耐药菌株MS-17-88的耐药机制。为实现这一目标，提取、测序并组装了基因组DNA。MS-17-88菌株基因组由5,178,226个碱基对组成，G+C含量为58.6%，它编码了几种AMR元件，包括……和……。对包括MS-17-88在内的大量该菌株的系统发育和耐药谱进行了评估。系统发育分析表明，MS-17-88与从中国鱼类中分离出的Ae5菌株以及从日本池塘水中分离出的ARB3菌株关系密切，表明这些菌株有共同的祖先。噬菌体元件分析显示，在53个该菌株基因组中，有58个完整、63个不完整和15个可疑的噬菌体元件。每个基因组的噬菌体元件平均数量为2.56个，MS-17-88菌株是具有已鉴定原噬菌体元件数量最多（各6个元件）的两个菌株之一。对53个该菌株基因组的各种抗生素耐药谱分析显示，所有基因组（100%）中都存在……和……。相比之下，在7.5%和1.8%的该菌株基因组中检测到……和……。近77%的菌株携带……，7.5%的菌株携带……。这一结果表明，与该菌株中的四环素抗性相比，磺胺类和氟苯尼考抗性基因的丰度和流行率较低。总体而言，本研究为53个该菌株基因组的耐药模式提供了见解，可为受该菌影响的鱼类的治疗选择提供参考。
