Department of Integrative Physiology, Shiga University of Medical Science, Otsu 520-2192, Japan.
Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki 444-8787, Japan.
Cereb Cortex. 2020 Nov 3;30(12):6415-6425. doi: 10.1093/cercor/bhaa200.
The origin and life-long fate of quiescent neural stem cells (NSCs) in the adult mammalian brain remain largely unknown. A few neural precursor cells in the embryonic brain elongate their cell cycle time and subsequently become quiescent postnatally, suggesting the possibility that life-long NSCs are selected at an early embryonic stage. Here, we utilized a GFP-expressing lentivirus to investigate the fate of progeny from individual lentivirus-infected NSCs by identifying the lentiviral integration site. Our data suggest that NSCs become specified to two or more lineages prior to embryonic day 13.5 in mice: one NSC lineage produces cells only for the cortex and another provides neurons to the olfactory bulb. The majority of neurosphere-forming NSCs in the adult brain are relatively dormant and generate very few cells, if any, in the olfactory bulb or cortex, and this NSC population could serve as a reservoir that is occasionally reactivated later in life.
静止神经干细胞（NSCs）在成年哺乳动物大脑中的起源和终身命运在很大程度上仍然未知。胚胎大脑中的一些神经前体细胞延长了它们的细胞周期时间，随后在出生后变得静止，这表明终身 NSCs 可能是在早期胚胎阶段被选择的。在这里，我们利用表达 GFP 的慢病毒，通过鉴定慢病毒整合位点来研究单个慢病毒感染的 NSCs 后代的命运。我们的数据表明，在小鼠中，NSCs 在胚胎第 13.5 天之前就已经被指定为两个或更多谱系：一个 NSC 谱系仅产生用于皮层的细胞，另一个则提供神经元到嗅球。成年大脑中大多数神经球形成 NSCs 相对休眠，很少产生任何细胞，如果有的话，在嗅球或皮层中，并且这个 NSC 群体可以作为一个储备库，在以后的生活中偶尔被重新激活。
