Key Lab of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China; Engineering Research Center of Chinese Medicine Resources, Ministry of Education, Beijing 100193, China.
Key Lab of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China; Engineering Research Center of Chinese Medicine Resources, Ministry of Education, Beijing 100193, China.
Chin J Nat Med. 2020 Aug;18(8):606-611. doi: 10.1016/S1875-5364(20)30072-8.
As abscisic acid (ABA) receptor, the pyrabactin resistance 1-like (PYR/PYL) protein (named PYL for simplicity) plays an important part to unveil the signal transduction of ABA and its regulatory mechanisms. Glycyrrhiza uralensis, a drought-tolerant medicinal plant, is a good model for the mechanism analysis of ABA response and active compound biosynthesis. However, knowledge about PYL family in G. uralensis remains largely unknown. Here, 10 PYLs were identified in G. uralensis genome. Characterization analysis indicated that PYLs in G. uralensis (GuPYLs) are relatively conserved. Phylogenetic analysis showed that GuPYL1-3 belongs to subfamily I, GuPYL4-6 and GuPYL10 belong to subfamily II and GuPYL7-9 belongs to subfamily III. In addition, transcriptome data presented various expression levels of GuPYLs under different exogenous ABA stresses. The expression pattern of GuPYLs was verified by Quantitative real-time polymerase chain reaction (qRT-PCR). The study proved that GuPYL4, GuPYL5, GuPYL8 and GuPYL9 genes are significantly up-regulated by ABA stress and the response process is dynamic. This study paves the way for elucidating the regulation mechanism of ABA signal to secondary metabolites and improving the cultivation and quality of G. uralensis using agricultural strategies.
作为脱落酸（ABA）受体，吡喃并[2,3-d]嘧啶类受体（PYR/PYL）蛋白（简称为 PYL）在揭示 ABA 的信号转导及其调控机制方面发挥着重要作用。甘草是一种耐旱的药用植物，是分析 ABA 反应和活性化合物生物合成机制的良好模型。然而，甘草中 PYL 家族的知识仍然知之甚少。本研究在甘草基因组中鉴定了 10 个 PYL。特征分析表明，甘草 PYLs（GuPYLs）相对保守。系统发育分析表明，GuPYL1-3 属于 I 亚家族，GuPYL4-6 和 GuPYL10 属于 II 亚家族，GuPYL7-9 属于 III 亚家族。此外，转录组数据显示不同外源 ABA 胁迫下 GuPYLs 的表达水平存在差异。通过定量实时聚合酶链反应（qRT-PCR）验证了 GuPYLs 的表达模式。研究证明，ABA 胁迫下 GuPYL4、GuPYL5、GuPYL8 和 GuPYL9 基因显著上调，且响应过程具有动态性。该研究为阐明 ABA 信号对次生代谢物的调控机制以及利用农业策略提高甘草的栽培和质量奠定了基础。
