Han Yuxuan, Hou Zhuoni, He Qiuling, Zhang Xuemin, Yan Kaijing, Han Ruilian, Liang Zongsuo
The Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.
Tasly R&D Institute, Tasly Holding Group Co., Ltd., Tianjin, China.
Front Genet. 2021 Oct 5;12:754237. doi: 10.3389/fgene.2021.754237. eCollection 2021.
gene family is one of the largest transcription factor families. It plays an important role in plant growth, metabolic, and environmental response. However, complete genome-wide investigation of gene family in remains unexplained. In this study, 66 putative genes in the genome of were identified. And their evolutionary classification, physicochemical properties, conserved domain, functional differentiation, and the expression level under different stress conditions were further analyzed. All the members were clustered into 13 subfamilies (A-K, M, and S). A total of 10 conserved motifs were found in GubZIP proteins. Members from the same subfamily shared highly similar gene structures and conserved domains. Tandem duplication events acted as a major driving force for the evolution of gene family in . Cis-acting elements and protein-protein interaction networks showed that in one subfamily are involved in multiple functions, while some from different subfamilies may share the same functional category. The miRNA network targeting GubZIPs showed that the regulation at the transcriptional level may affect protein-protein interaction networks. We suspected that domain-mediated interactions may categorize a protein family into subfamilies in G. uralensis. Furthermore, the tissue-specific gene expression patterns of GubZIPs were analyzed using the public RNA-seq data. Moreover, gene expression level of 66 bZIP family members under abiotic stress treatments was quantified by using qRT-PCR. The results of this study may serve as potential candidates for functional characterization in the future.
基因家族是最大的转录因子家族之一。它在植物生长、代谢和环境响应中发挥着重要作用。然而，关于[物种名称]基因家族的全基因组完整研究仍未得到充分解释。在本研究中，我们在[物种名称]的基因组中鉴定出了66个假定的[基因名称]基因。并进一步分析了它们的进化分类、理化性质、保守结构域、功能分化以及在不同胁迫条件下的表达水平。所有成员被聚类为13个亚家族（A - K、M和S）。在古bZIP蛋白中总共发现了10个保守基序。来自同一亚家族的成员具有高度相似的基因结构和保守结构域。串联重复事件是[物种名称]中[基因名称]基因家族进化的主要驱动力。顺式作用元件和蛋白质 - 蛋白质相互作用网络表明，一个亚家族中的[基因名称]参与多种功能，而一些来自不同亚家族的[基因名称]可能共享相同的功能类别。靶向古bZIPs的miRNA网络表明，转录水平的调控可能影响蛋白质 - 蛋白质相互作用网络。我们推测，结构域介导的相互作用可能将一个蛋白质家族在乌拉尔甘草中分类为不同的亚家族。此外，利用公开的RNA - seq数据分析了古bZIPs的组织特异性基因表达模式。而且，通过qRT - PCR定量了66个bZIP家族成员在非生物胁迫处理下的基因表达水平。本研究结果可能为未来的功能表征提供潜在的候选基因。
