Laboratory of Electrophysiology of Epithelial Tissue and Skin, Department of Pathobiochemistry and Clinical Chemistry, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Poland.
Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Poland.
Biomed Pharmacother. 2020 Sep;129:110502. doi: 10.1016/j.biopha.2020.110502. Epub 2020 Jul 17.
Platinum-based drugs, used in treating tumors, cause numerous undesirable effects in patients, like neuropathic pain, hypersensitivity, reddening, pruritus and rash. Changes in Na transport modify local osmolality and contribute to the initiation of hypersensitivity and allergy. They are also associated with stimulation of C-fibres and hyperalgesia. Cl transport is essential for regulation of sweat composition and the migration of immunocompetent cells. The aim of the conducted study was to assess the effect of a cisplatin solution on the electrophysiological parameters of the isolated rabbit skin specimens. The difference in transepithelial electrical potential (PD) and resistance (R) in stationary conditions and during 15 s mechanical-chemical stimulation (PDmin and PDmax), were measured. Measurement of R revealed that tissue samples were live, and their permeability to ions were stable. Control specimens had PD -0.22 mV (median). The PD of specimens treated by cisplatin was -0.55 mV (median), to for cisplatin and bumetanide 0 mV (median). Treatment with cisplatin did not change the continuous transport of Na and K ions, but did change that of Cl ions. Stimulation of samples with the transport blockers of Cl, Na and both induced repeatable and measurable reactions in the transport of the appropriate ions. It was shown that absorption of Na ions and release of Cl ions was intensified than in the untreated specimens. It was proven in the study that cisplatin influences the Na and Cl transport in the skin cells. Restoring the balance in ion flow can prevent side effects of use cisplatin-based drugs.
铂类药物在治疗肿瘤时会给患者带来许多不良反应，如神经病理性疼痛、超敏反应、发红、瘙痒和皮疹。钠转运的变化会改变局部渗透压，并有助于引发超敏反应和过敏。它们还与 C 纤维的刺激和痛觉过敏有关。氯转运对于调节汗液成分和免疫活性细胞的迁移至关重要。本研究旨在评估顺铂溶液对离体兔皮标本电生理参数的影响。在静止状态和 15 秒机械化学刺激期间（PDmin 和 PDmax）测量跨上皮电潜力（PD）和电阻（R）的差异。R 的测量表明组织样本是活的，其离子通透性稳定。对照标本的 PD 为-0.22 mV（中位数）。顺铂处理标本的 PD 为-0.55 mV（中位数），顺铂和布美他尼为 0 mV（中位数）。顺铂处理并未改变 Na 和 K 离子的连续转运，但改变了 Cl 离子的转运。用 Cl、Na 和两者的转运阻断剂刺激样本，会引起相应离子转运的可重复和可测量的反应。结果表明，吸收 Na 离子和释放 Cl 离子的速度比未经处理的标本更快。研究表明，顺铂会影响皮肤细胞中的 Na 和 Cl 转运。恢复离子流的平衡可以预防使用基于顺铂的药物的副作用。
