Department of Pathobiochemistry and Clinical Chemistry, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 85-094 Bydgoszcz, Poland.
Department of Medical Biology and Biochemistry, Faculty of Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 85-092 Bydgoszcz, Poland.
Int J Mol Sci. 2024 Sep 6;25(17):9670. doi: 10.3390/ijms25179670.
Retinoids are known to improve the condition of the skin. Transepithelial transport of sodium and chloride ions is important for proper skin function. So far, the effect of applying vitamin A preparations to the skin on ion transport has not been evaluated. In the study, electrophysiological parameters, including transepithelial electric potential (PD) and transepithelial resistance (R), of rabbit skin specimens after 24 h exposure to retinol ointment (800 mass units/g) were measured in a modified Ussing chamber. The R of the fragments incubated with retinol was significantly different than that of the control skin samples incubated in iso-osmotic Ringer solution. For the controls, the PD values were negative, whereas the retinol-treated specimens revealed positive PD values. Mechanical-chemical stimulation with the use of inhibitors of the transport of sodium (amiloride) or chloride (bumetanide) ions revealed specific changes in the maximal and minimal PD values measured for the retinol-treated samples. Retinol was shown to slightly modify the transport pathways of sodium and chloride ions. In particular, an intensification of the chloride ion secretion from keratinocytes was observed. The proposed action may contribute to deep hydration and increase skin tightness, limiting the action of other substances on its surface.
视黄醇类物质已知可改善皮肤状况。钠离子和氯离子的跨上皮转运对于皮肤的正常功能很重要。到目前为止，还没有评估将维生素 A 制剂应用于皮肤对离子转运的影响。在这项研究中，使用改良的 Ussing 室测量了兔皮肤标本在 24 小时暴露于视黄醇软膏（800 质量单位/g）后的电生理学参数，包括跨上皮电位（PD）和跨上皮电阻（R）。与在等渗林格氏溶液中孵育的对照皮肤样本相比，用视黄醇孵育的片段的 R 明显不同。对于对照，PD 值为负值，而用视黄醇处理的标本则显示出正 PD 值。使用钠离子（氨苯蝶啶）或氯离子（布美他尼）转运抑制剂进行机械化学刺激，显示出针对视黄醇处理样本测量的最大和最小 PD 值的特定变化。视黄醇可轻微改变钠离子和氯离子的转运途径。特别是，观察到角质形成细胞中氯离子分泌的增强。所提出的作用可能有助于深度水合并增加皮肤紧致度，限制其他物质在其表面的作用。
