Laboratory of Electrophysiology of Epithelial Tissue and Skin, Department of Pathobiochemistry and Clinical Chemistry, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Torun, M. Skłodowskiej-Curie 9, 85-094, Bydgoszcz, Poland.
Department of Medical Biology and Biochemistry, Faculty of Medicine, Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Torun, Bydgoszcz, Poland.
Sci Rep. 2020 Oct 22;10(1):18094. doi: 10.1038/s41598-020-75275-3.
The measurement of electric potential and resistance reflect the transport of sodium and chloride ions which take place in keratinocytes and is associated with skin response to stimuli arising from external and internal environment. The aim of the study was to assess changes in electrical resistance and the transport of chloride and sodium ions, under iso-osmotic conditions and following the use of inhibitors affecting these ions' transport, namely amiloride (A) and bumetanide (B). The experiment was performed on 104 fragments of rabbit skin, divided into three groups: control (n = 35), A-inhibited sodium transport (n = 33) and B-inhibited chloride transport (n = 36). Measurement of electrical resistance (R) and electrical potential (PD) confirmed tissue viability during the experiment, no statistically significant differences in relation to control conditions were noted. The minimal and maximal PD measured during stimulation confirmed the repeatability of the recorded reactions to the mechanical and mechanical-chemical stimulus for all examined groups. Measurement of PD during stimulation showed differences in the transport of sodium and chloride ions in each of the analyzed groups relative to the control. The statistical analysis of the PD measured in stationary conditions and during mechanical and/or mechanical-chemical stimulation proved that changes in sodium and chloride ion transport constitute the physiological response of keratinocytes to changes in environmental conditions for all applied experimental conditions. Assessment of transdermal ion transport changes may be a useful tool for assessing the skin condition with tendency to pain hyperactivity and hypersensitivity to xenobiotics.
测量电潜能和电阻反映了钠离子和氯离子的转运，这些转运发生在角质形成细胞中，与皮肤对外界和内部环境刺激的反应有关。本研究的目的是评估在等渗条件下以及使用影响这些离子转运的抑制剂（即阿米洛利（A）和布美他尼（B））后，电电阻和氯离子、钠离子转运的变化。实验在 104 个兔皮碎片上进行，分为三组：对照组（n=35）、A 抑制钠离子转运组（n=33）和 B 抑制氯离子转运组（n=36）。测量电阻（R）和电潜能（PD）证实了实验过程中组织的活力，与对照条件相比，没有统计学意义上的差异。在刺激过程中测量的最小和最大 PD 证实了所有检查组对机械和机械化学刺激的记录反应具有可重复性。在刺激过程中 PD 的测量表明，在每个分析组中，钠离子和氯离子的转运与对照组相比存在差异。在静止条件以及机械和/或机械化学刺激下测量的 PD 的统计分析证明，钠离子和氯离子转运的变化构成了角质形成细胞对所有应用实验条件下环境条件变化的生理反应。评估经皮离子转运变化可能是评估皮肤状况的有用工具，这种皮肤状况具有疼痛过度活跃和对外源性物质过敏的倾向。
