Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
Graduate School, Chiang Mai University, Chiang Mai, 50200, Thailand.
Sci Rep. 2020 Aug 7;10(1):13379. doi: 10.1038/s41598-020-70214-8.
This study aimed to investigate the effects of morin on cerebral damage and blood-brain barrier (BBB) integrity in a middle cerebral artery occlusion (MCAO) and reperfusion model. Wistar rats were exposed to MCAO for 2 h, followed by reperfusion. Thirty mg/kg of morin was administered via intraperitoneal injection at the different time points: before ischemia, during ischemia, and at reperfusion. The rats were divided into five groups, including sham, vehicle, and three groups of morin. Twenty-four hours after reperfusion, the rats were tested for neurological deficits, and the brains were harvested to assess brain damage. In addition, brains were harvested 72 h to determine BBB disruption. We found that morin significantly reduced reactive oxygen species production and lipid peroxidation. It also decreased inflammation via reducing the expression of Toll-like receptor 4, nuclear factor kappa-beta. Morin ameliorated cerebral damage and reduced apoptosis through decreasing the cerebral infarct size, including apoptotic cell death. Moreover, morin decreased the BBB damage via reducing Evans blue extravasation, neutrophil infiltration, and increasing tight junction protein expression. Therefore, morin protected against cerebral and BBB damage by attenuating oxidative stress, inflammation, and apoptosis in MCAO and reperfusion models.
这项研究旨在探讨桑色素对大脑损伤和血脑屏障（BBB）完整性的影响，建立大脑中动脉阻塞（MCAO）和再灌注模型。将 Wistar 大鼠暴露于 MCAO 中 2 小时，然后再灌注。在不同时间点通过腹腔注射给予 30mg/kg 的桑色素：缺血前、缺血期间和再灌注期间。将大鼠分为 5 组，包括假手术组、载体组和 3 组桑色素组。再灌注后 24 小时，对大鼠进行神经功能缺损测试，并取出大脑评估脑损伤。此外，在 72 小时时取出大脑以确定 BBB 破坏。我们发现，桑色素可显著减少活性氧的产生和脂质过氧化。它还通过降低 Toll 样受体 4、核因子 kappa-beta 的表达来减少炎症。桑色素通过减少脑梗死面积，包括凋亡细胞死亡，减轻了脑损伤和细胞凋亡。此外，桑色素通过减少 Evans 蓝外渗、中性粒细胞浸润和增加紧密连接蛋白的表达来减少 BBB 损伤。因此，桑色素通过减轻 MCAO 和再灌注模型中的氧化应激、炎症和细胞凋亡来保护大脑和 BBB 免受损伤。
