Gentile J M, Heydenburg D, Hertel L, Plewa M J
Mutat Res. 1987 Jul-Aug;191(3-4):151-5. doi: 10.1016/0165-7992(87)90146-1.
Mutagenic activity of the promutagens 2-aminofluorene (2AF) and a contaminant of 4-nitro-o-phenylenediamine (NOP-X) was followed in Ames Salmonella strain TA98 following metabolism by cotton and carrot cell suspension cultures using the plant cell/microbe coincubation assay. Both cell lines were capable of activating each chemical. However, activation capacities of the cell lines differed relative to their respective stage of growth when used. For 2AF activation early-log phase cotton cells and mid-log phase carrot cells proved superior while mid-log phase cotton cells and stationary phase carrot cells proved superior for NOP-X activation. These data indicate that the phase of the growth cycle at which plant cells are harvested can significantly affect their promutagen activation potential.
采用植物细胞/微生物共培养试验，在艾姆斯沙门氏菌TA98菌株中追踪前诱变剂2-氨基芴（2AF）和4-硝基邻苯二胺污染物（NOP-X）经棉花和胡萝卜细胞悬浮培养物代谢后的诱变活性。两种细胞系均能够激活每种化学物质。然而，当使用时，细胞系的激活能力相对于其各自的生长阶段有所不同。对于2AF激活，对数早期的棉花细胞和对数中期的胡萝卜细胞表现更优，而对于NOP-X激活，对数中期的棉花细胞和稳定期的胡萝卜细胞表现更优。这些数据表明，收获植物细胞的生长周期阶段会显著影响其前诱变剂激活潜力。
