Higgins G A, Lewis D A, Bahmanyar S, Goldgaber D, Gajdusek D C, Young W G, Morrison J H, Wilson M C
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Proc Natl Acad Sci U S A. 1988 Feb;85(4):1297-301. doi: 10.1073/pnas.85.4.1297.
We have mapped the neuroanatomical distribution of amyloid-beta-protein mRNA within neuronal subpopulations of the hippocampal formation in the cynomolgus monkey (Macaca fascicularis), normal aged human, and patients with Alzheimer disease. Amyloid-beta-protein mRNA appears to be expressed in all hippocampal neurons, but at different levels of abundance. In the central nervous system of monkey and normal aged human, image analysis shows that neurons of the dentate gyrus and cornu Ammonis fields contain a 2.5-times-greater hybridization signal than is present in neurons of the subiculum and entorhinal cortex. In contrast, in the Alzheimer disease hippocampal formation, the levels of amyloid-beta-protein mRNA in the cornu Ammonis field 3 and parasubiculum are equivalent. These findings suggest that within certain neuronal subpopulations cell type-specific regulation of amyloid-beta-protein gene expression may be altered in Alzheimer disease.
我们已绘制出食蟹猴（猕猴）、正常老年人以及阿尔茨海默病患者海马结构神经元亚群中β-淀粉样蛋白mRNA的神经解剖分布。β-淀粉样蛋白mRNA似乎在所有海马神经元中均有表达，但丰度水平不同。在猴和正常老年人的中枢神经系统中，图像分析显示齿状回和海马角神经元的杂交信号比海马下托和内嗅皮质神经元中的信号强2.5倍。相比之下，在阿尔茨海默病患者的海马结构中，海马角3区和副海马下托中β-淀粉样蛋白mRNA的水平相当。这些发现表明，在某些神经元亚群中，阿尔茨海默病可能会改变β-淀粉样蛋白基因表达的细胞类型特异性调控。
