Institute of Agrobiological Sciences, NARO (NIAS), Tsukuba, Japan.
Research Institute for Biological Sciences, Okayama Prefectural Technology Center for Agriculture, Forestry, and Fisheries, Okayama, Japan.
Plant Cell Rep. 2020 Nov;39(11):1493-1503. doi: 10.1007/s00299-020-02578-0. Epub 2020 Aug 9.
The overexpression of rice BSR2 would offer a simple and effective strategy to protect plants from multiple devastating diseases in tomato and Arabidopsis. Many devastating plant diseases are caused by pathogens possessing a wide host range. Fungal Botrytis cinerea and Rhizoctonia solani, as well as bacterial Pseudomonas syringae and Ralstonia pseudosolanacearum are four such pathogens that infect hundreds of plant species, including agronomically important crops, and cause serious diseases, leading to severe economic losses. However, reports of genes that can confer resistance to broad host-range pathogens via traditional breeding methods are currently limited. We previously reported that Arabidopsis plants overexpressing rice BROAD-SPECTRUM RESISTANCE2 (BSR2/CYP78A15) showed tolerance not only to bacterial P. syringae pv. tomato DC3000 but also to fungal Colletotrichum higginsianum and R. solani. Rice plants overexpressing BSR2 displayed tolerance to two R. solani anastomosis groups. In the present study, first, BSR2-overexpressing (OX) Arabidopsis plants were shown to be additionally tolerant to B. cinerea, R. solani, and R. pseudosolanacearum. Next, tomato 'Micro-Tom' was used as a model to determine whether such tolerance by BSR2 can be introduced into dicot crops to prevent infection from pathogens possessing wide host range. BSR2-OX tomato displayed broad-spectrum disease tolerance to fungal B. cinerea and R. solani, as well as to bacterial P. syringae and R. pseudosolanacearum. Additionally, undesirable traits such as morphological changes were not detected. Thus, BSR2 overexpression can offer a simple and effective strategy to protect crops from multiple destructive diseases.
水稻 BSR2 的过表达为保护番茄和拟南芥中的多种破坏性疾病提供了一种简单而有效的策略。许多破坏性植物疾病是由具有广泛宿主范围的病原体引起的。真菌灰葡萄孢和立枯丝核菌，以及细菌丁香假单胞菌和茄青枯假单胞菌是四种具有如此广泛宿主范围的病原体，它们感染包括农业上重要作物在内的数百种植物，并导致严重疾病，造成严重的经济损失。然而，通过传统的育种方法报告能够赋予对广泛宿主范围病原体抗性的基因的报道目前是有限的。我们之前报道过，拟南芥植物过表达水稻 BROAD-SPECTRUM RESISTANCE2 (BSR2/CYP78A15) 不仅对细菌丁香假单胞菌 pv.番茄 DC3000 具有耐受性，而且对真菌炭疽菌和立枯丝核菌也具有耐受性。过表达 BSR2 的水稻植株对两个立枯丝核菌吻合组表现出耐受性。在本研究中，首先，过表达 BSR2 的拟南芥植物被证明对灰葡萄孢、立枯丝核菌和茄青枯假单胞菌具有额外的耐受性。接下来，以番茄“Micro-Tom”为模型，确定 BSR2 的这种耐受性是否可以引入双子叶作物中，以防止感染具有广泛宿主范围的病原体。BSR2-OX 番茄对真菌灰葡萄孢和立枯丝核菌以及细菌丁香假单胞菌和茄青枯假单胞菌表现出广谱的疾病耐受性。此外，没有检测到不良性状，如形态变化。因此，BSR2 的过表达可以为保护作物免受多种破坏性疾病提供一种简单而有效的策略。
