Cell Therapy Innovation Center, Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan, ROC.
Institute of Pharmacology, School of Pharmaceutical Science, National Yang-Ming University, Taipei, Taiwan, ROC.
J Chin Med Assoc. 2020 Aug;83(8):725-732. doi: 10.1097/JCMA.0000000000000387.
The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused severe pneumonia at December 2019. Since then, it has been wildly spread from Wuhan, China, to Asia, European, and United States to become the pandemic worldwide. Now coronavirus disease 2019 were globally diagnosed over 3 084 740 cases with mortality of 212 561 toll. Current reports variants are found in SARS-CoV-2, majoring in functional ribonucleic acid (RNA) to transcribe into structural proteins as transmembrane spike (S) glycoprotein and the nucleocapsid (N) protein holds the virus RNA genome; the envelope (E) and membrane (M) alone with spike protein form viral envelope. The nonstructural RNA genome includes ORF1ab, ORF3, ORF6, 7a, 8, and ORF10 with highly conserved information for genome synthesis and replication in ORF1ab.
We apply genomic alignment analysis to observe SARS-CoV-2 sequences from GenBank (http://www.ncbi.nim.nih.gov/genebank/): MN 908947 (China, C1); MN985325 (United States: WA, UW); MN996527 (China, C2); MT007544 (Australia: Victoria, A1); MT027064 (United States: CA, UC); MT039890 (South Korea, K1); MT066175 (Taiwan, T1); MT066176 (Taiwan, T2); LC528232 (Japan, J1); and LC528233 (Japan, J2) and Global Initiative on Sharing All Influenza Data database (https://www.gisaid.org). We adopt Multiple Sequence Alignments web from Clustalw (https://www.genome.jp/tools-bin/clustalw) and Geneious web (https://www.geneious.com.
We analyze database by genome alignment search for nonstructural ORFs and structural E, M, N, and S proteins. Mutations in ORF1ab, ORF3, and ORF6 are observed; specific variants in spike region are detected.
We perform genomic analysis and comparative multiple sequence of SARS-CoV-2. Large scaling sequence alignments trace to localize and catch different mutant strains in United possibly to transmit severe deadly threat to humans. Studies about the biological symptom of SARS-CoV-2 in clinic animal and humans will be applied and manipulated to find mechanisms and shield the light for understanding the origin of pandemic crisis.
严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的爆发导致 2019 年 12 月发生严重肺炎。此后，它从中国武汉迅速蔓延到亚洲、欧洲和美国，成为全球大流行。目前，全球已诊断出超过 3084740 例 2019 年冠状病毒病病例，死亡 212561 人。目前的报告显示，SARS-CoV-2 中存在变异株，主要是功能核糖核酸（RNA）转录为结构蛋白，如跨膜刺突（S）糖蛋白和核衣壳（N）蛋白，携带病毒 RNA 基因组；包膜（E）和膜（M）与刺突蛋白一起形成病毒包膜。非结构 RNA 基因组包括 ORF1ab、ORF3、ORF6、7a、8 和 ORF10，其中包含基因组合成和复制的高度保守信息 ORF1ab。
我们应用基因组比对分析观察来自 GenBank（http://www.ncbi.nlm.nih.gov/genebank/）的 SARS-CoV-2 序列：MN908947（中国，C1）；MN985325（美国：WA、UW）；MN996527（中国，C2）；MT007544（澳大利亚：维多利亚，A1）；MT027064（美国：CA、UC）；MT039890（韩国，K1）；MT066175（中国台湾，T1）；MT066176（中国台湾，T2）；LC528232（日本，J1）和 LC528233（日本，J2）和全球流感共享数据倡议数据库（https://www.gisaid.org）。我们采用来自 Clustalw（https://www.genome.jp/tools-bin/clustalw）的 Multiple Sequence Alignments web 和 Geneious web（https://www.geneious.com）进行数据库多序列比对分析。
我们通过基因组比对搜索非结构 ORFs 和结构 E、M、N 和 S 蛋白进行数据库分析。在 ORF1ab、ORF3 和 ORF6 中观察到突变，在刺突区检测到特定的变异体。
我们对 SARS-CoV-2 进行了基因组分析和比较多序列分析。大规模序列比对追踪到美国的不同突变株，并可能对人类传播严重致命威胁。关于 SARS-CoV-2 在临床动物和人类中的生物学症状的研究将被应用和操作，以找到机制，并为了解大流行危机的起源提供启示。
