Aklilu Erkihun, Raman Kausalya
Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Locked Bag 36, Kota Bharu, Kelantan, Malaysia.
Int J Microbiol. 2020 Jul 28;2020:8853582. doi: 10.1155/2020/8853582. eCollection 2020.
This study was conducted to detect the presence of colistin-resistant (. ) in raw chicken meat and bean sprouts collected from local markets and to determine the antimicrobial resistance patterns of the . isolates. A total of 100 samples, comprised of 50 raw chicken meat and 50 bean sprouts, were collected and processed. Kirby-Bauer method was used to determine the antimicrobial resistance patterns, and PCR amplification was used to detect . species-specific and colistin resistance (-1 and -2) genes. The results showed that 52.1% (12/23) of the . isolated from raw chicken meat were positive for the colistin resistance encoding gene, -1, whereas all the . isolates from bean sprouts were negative for colistin resistance encoding genes. The findings show that chicken meat contaminated with colistin-resistant . may pose public health risk to the consumers. Hence, prudent usage of antibiotics and hygienic handling of food items helps to prevent and combat the risks of spreading of colistin-resistant . and the public health risks it may pose. More comprehensive and large-scale studies focusing on all the possible sources of colistin-resistant . are recommended.
本研究旨在检测从当地市场采集的生鸡肉和豆芽中耐黏菌素的[具体细菌名称未给出]的存在情况，并确定这些[具体细菌名称未给出]分离株的抗菌耐药模式。总共采集并处理了100个样本，其中包括50份生鸡肉和50份豆芽。采用 Kirby-Bauer 方法确定抗菌耐药模式，并通过 PCR 扩增检测[具体细菌名称未给出]的种特异性和耐黏菌素抗性（mcr-1和mcr-2）基因。结果显示，从生鸡肉中分离出的[具体细菌名称未给出]中有52.1%（12/23）对编码耐黏菌素的基因mcr-1呈阳性，而从豆芽中分离出的所有[具体细菌名称未给出]分离株对编码耐黏菌素的基因均呈阴性。研究结果表明，被耐黏菌素的[具体细菌名称未给出]污染的鸡肉可能会给消费者带来公共卫生风险。因此，谨慎使用抗生素和对食品进行卫生处理有助于预防和应对耐黏菌素的[具体细菌名称未给出]传播风险及其可能带来的公共卫生风险。建议开展更全面、大规模的研究，关注耐黏菌素的[具体细菌名称未给出]的所有可能来源。
