Karim Md Rezaul, Zakaria Zunita, Hassan Latiffah, Faiz Nik Mohd, Ahmad Nur Indah
Department of Veterinary Pathology & Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang, Selangor, Malaysia.
Bangladesh Livestock Research Institute, Dhaka, Bangladesh.
Front Microbiol. 2023 Aug 3;14:1208314. doi: 10.3389/fmicb.2023.1208314. eCollection 2023.
The advent of antimicrobials-resistant (AMR), including colistin-resistant bacteria, poses a significant challenge to animal and human health, food safety, socio-economic growth, and the global environment. This study aimed to ascertain the colistin resistance prevalence and molecular mechanisms of colistin resistance in . The colistin resistance was determined using broth microdilution assay, PCR; and Sanger sequencing of genes responsible for colistin resistance in ( = 627), including (436) spp. ( = 140), and ( = 51), obtained from chicken and chicken meats. Out of 627 , 8.6% of isolates exhibited colistin resistance phenotypically. Among these colistin resistant isolates, 9.3% ( = 37) were isolated from chicken meat, 7.2% ( = 11) from the cloacal swab of chicken and 7.9% ( = 6) from the litter samples. Overall, 12.96% of colistin-resistant isolates were positive with genes, in which and genes were determined in 11.11% and 1.85% of colistin-resistant isolates, respectively. The isolates obtained from chicken meats, cloacal swabs and litter samples were found positive for , and spp. originated from the chicken meat sample was observed with , whereas no genes were observed in strains isolated from any of the collected samples. The other colistin resistance genes, including and were not detected in the studied samples. The and genes were sequenced and found to be 100% identical to the and gene sequences available in the NCBI database. This is the first report of colistin resistance gene in Malaysia which could portend the emergence of harboring bacterial strains for infection. Further studies are needed to characterize the harbouring bacteria for the determination of plasmid associated with gene.
包括耐黏菌素细菌在内的抗菌药物耐药性（AMR）的出现，对动物和人类健康、食品安全、社会经济增长以及全球环境构成了重大挑战。本研究旨在确定鸡肉和鸡肉制品中耐黏菌素的流行情况以及耐黏菌素的分子机制。使用肉汤微量稀释法、PCR以及对627株从鸡肉和鸡肉制品中分离得到的菌株（包括436株鸡源菌株、140株其他物种菌株和51株其他菌株）中负责耐黏菌素的基因进行Sanger测序来确定耐黏菌素情况。在627株菌株中，8.6%的分离株表现出耐黏菌素表型。在这些耐黏菌素分离株中，9.3%（n = 37）分离自鸡肉，7.2%（n = 11）分离自鸡泄殖腔拭子，7.9%（n = 6）分离自垫料样本。总体而言，12.96%的耐黏菌素分离株 基因呈阳性，其中mcr - 1和mcr - 2基因分别在11.11%和1.85%的耐黏菌素分离株中被检测到。从鸡肉、泄殖腔拭子和垫料样本中获得的分离株被发现mcr - 1、mcr - 2和mcr - 3基因呈阳性，源自鸡肉样本的菌株观察到mcr - 4基因，而从任何收集样本中分离的大肠杆菌菌株均未观察到mcr - 5基因。在所研究的样本中未检测到其他耐黏菌素基因，包括mcr - 6和mcr - 7。对mcr - 1和mcr - 2基因进行测序，发现与NCBI数据库中可用的mcr - 1和mcr - 2基因序列100%相同。这是马来西亚关于耐黏菌素mcr基因的首次报告，这可能预示着携带该基因的感染性细菌菌株的出现。需要进一步研究来鉴定携带mcr基因的细菌，以确定与mcr基因相关的质粒。
