• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

骨肉瘤中组蛋白H4赖氨酸20三甲基化的缺失与组蛋白甲基转移酶SUV420H2的异常表达有关。

Loss of histone H4 lysine 20 trimethylation in osteosarcoma is associated with aberrant expression ofhistone methyltransferase SUV420H2.

作者信息

Piao Lianhua, Yuan Xiaofeng, Wang Luhui, Xu Xiaoshuang, Zhuang Ming, Li Jinggao, Kong Ren, Liu Zhiwei

机构信息

Institute of Bioinformatics and Medical Engineering, Jiangsu University of Technology, Changzhou, Jiangsu 213001, P.R. China.

Department of Orthopaedics, The Third Affiliated Hospital of SooChow University, Changzhou, Jiangsu 213000, P.R. China.

出版信息

Oncol Lett. 2020 Oct;20(4):26. doi: 10.3892/ol.2020.11887. Epub 2020 Jul 16.

DOI:10.3892/ol.2020.11887
PMID:32774499
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7406877/
Abstract

Epigenetic modifications of histones have crucial roles in various types of cancers. The aberrant trimethylation of histone H4 at lysine 20 (H4K20) has been implicated in carcinogenesis. At present, the status of trimethylation at H4k20 (H4K20me3) in osteosarcoma (OS), the predominant bone cancer in humans, is unknown. In the present study, a genome-wide decrease was observed in H4K20me3 levels in OS tissues and cell lines. Reduced levels of lysine methyltransferase 5C (SUV420H2), the histone methyltranferase responsible for modification of H4K20me3, was also observed in OS cells with the associated loss of H4K20me3. Furthermore, a total of 507 SUV420H2-regulated genes were identified through RNA-seq and a number of candidate genes were further validated. Bioinformatic analysis revealed an association between SUV420H2 and multiple signaling pathway, including the mitogen-activated protein kinase, P53, transforming growth factor and the ErbB pathways. These results demonstrated that there are aberrant levels of H4K20me3 and SUV420H2 in OS, and highlighted H4K20me3 as a candidate biomarker for the early detection of OS.

摘要

组蛋白的表观遗传修饰在各类癌症中起着关键作用。组蛋白H4赖氨酸20位点（H4K20）的异常三甲基化与癌症发生有关。目前，在人类主要的骨癌骨肉瘤（OS）中，H4K20位点的三甲基化状态（H4K20me3）尚不清楚。在本研究中，观察到骨肉瘤组织和细胞系中H4K20me3水平在全基因组范围内降低。在骨肉瘤细胞中也观察到负责H4K20me3修饰的组蛋白甲基转移酶赖氨酸甲基转移酶5C（SUV420H2）水平降低，同时伴有H4K20me3的缺失。此外，通过RNA测序共鉴定出507个受SUV420H2调控的基因，并对一些候选基因进行了进一步验证。生物信息学分析揭示了SUV420H2与多种信号通路之间的关联，包括丝裂原活化蛋白激酶、P53、转化生长因子和表皮生长因子受体（ErbB）信号通路。这些结果表明，骨肉瘤中存在异常水平的H4K20me3和SUV420H2，并突出了H4K20me3作为骨肉瘤早期检测候选生物标志物的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddf1/7406877/2dfd4d8fe84a/ol-20-04-11887-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddf1/7406877/c9add7eeebbf/ol-20-04-11887-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddf1/7406877/d5b553477116/ol-20-04-11887-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddf1/7406877/f6178c9117ea/ol-20-04-11887-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddf1/7406877/5b77164d70fe/ol-20-04-11887-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddf1/7406877/50a8c7297406/ol-20-04-11887-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddf1/7406877/2dfd4d8fe84a/ol-20-04-11887-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddf1/7406877/c9add7eeebbf/ol-20-04-11887-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddf1/7406877/d5b553477116/ol-20-04-11887-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddf1/7406877/f6178c9117ea/ol-20-04-11887-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddf1/7406877/5b77164d70fe/ol-20-04-11887-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddf1/7406877/50a8c7297406/ol-20-04-11887-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddf1/7406877/2dfd4d8fe84a/ol-20-04-11887-g05.jpg

相似文献

1
Loss of histone H4 lysine 20 trimethylation in osteosarcoma is associated with aberrant expression ofhistone methyltransferase SUV420H2.骨肉瘤中组蛋白H4赖氨酸20三甲基化的缺失与组蛋白甲基转移酶SUV420H2的异常表达有关。
Oncol Lett. 2020 Oct;20(4):26. doi: 10.3892/ol.2020.11887. Epub 2020 Jul 16.
2
Loss of histone H4K20 trimethylation predicts poor prognosis in breast cancer and is associated with invasive activity.组蛋白H4K20三甲基化缺失预示乳腺癌预后不良，并与侵袭活性相关。
Breast Cancer Res. 2014 Jun 22;16(3):R66. doi: 10.1186/bcr3681.
3
A dual role for the histone methyltransferase PR-SET7/SETD8 and histone H4 lysine 20 monomethylation in the local regulation of RNA polymerase II pausing.组蛋白甲基转移酶 PR-SET7/SETD8 及其组蛋白 H4 赖氨酸 20 单甲基化在调控 RNA 聚合酶 II 暂停中的双重作用。
J Biol Chem. 2014 Mar 14;289(11):7425-37. doi: 10.1074/jbc.M113.520783. Epub 2014 Jan 23.
4
SUV420H2 suppresses breast cancer cell invasion through down regulation of the SH2 domain-containing focal adhesion protein tensin-3.SUV420H2通过下调含SH2结构域的粘着斑蛋白张力蛋白3来抑制乳腺癌细胞的侵袭。
Exp Cell Res. 2015 May 15;334(1):90-9. doi: 10.1016/j.yexcr.2015.03.010. Epub 2015 Mar 23.
5
Impact of histone methyltransferase SUV420H2 in breast cancer.组蛋白甲基转移酶SUV420H2在乳腺癌中的作用
Biomed Rep. 2020 Oct;13(4):29. doi: 10.3892/br.2020.1336. Epub 2020 Jul 23.
6
SUV420H2-mediated H4K20 trimethylation enforces RNA polymerase II promoter-proximal pausing by blocking hMOF-dependent H4K16 acetylation.SUV420H2 介导的 H4K20 三甲基化通过阻断 hMOF 依赖性 H4K16 乙酰化来强制 RNA 聚合酶 II 启动子近端暂停。
Mol Cell Biol. 2011 Apr;31(8):1594-609. doi: 10.1128/MCB.00524-10. Epub 2011 Feb 14.
7
H4K20me3 methyltransferase SUV420H2 shapes the chromatin landscape of pluripotent embryonic stem cells.H4K20me3 甲基转移酶 SUV420H2 塑造了多能胚胎干细胞的染色质景观。
Development. 2020 Dec 4;147(23):dev188516. doi: 10.1242/dev.188516.
8
Cross-species Analyses Unravel the Complexity of H3K27me3 and H4K20me3 in the Context of Neural Stem Progenitor Cells.跨物种分析揭示神经干细胞祖细胞背景下H3K27me3和H4K20me3的复杂性。
Neuroepigenetics. 2016 Jun;6:10-25. doi: 10.1016/j.nepig.2016.04.001. Epub 2016 May 3.
9
Loss of histone H4K20 trimethylation occurs in preneoplasia and influences prognosis of non-small cell lung cancer.组蛋白H4K20三甲基化缺失发生在肿瘤形成前，并影响非小细胞肺癌的预后。
Clin Cancer Res. 2008 Nov 15;14(22):7237-45. doi: 10.1158/1078-0432.CCR-08-0869. Epub 2008 Oct 30.
10
Comparative analyses of SUV420H1 isoforms and SUV420H2 reveal differences in their cellular localization and effects on myogenic differentiation.SUV420H1 同种型和 SUV420H2 的比较分析揭示了它们在细胞定位和对成肌分化影响方面的差异。
PLoS One. 2010 Dec 29;5(12):e14447. doi: 10.1371/journal.pone.0014447.

引用本文的文献

1
Epigenetic Modifications in Osteosarcoma: Mechanisms and Therapeutic Strategies.骨肉瘤中的表观遗传修饰：机制与治疗策略
Life (Basel). 2025 Jul 28;15(8):1202. doi: 10.3390/life15081202.
2
Tumor-suppressive functions of protein lysine methyltransferases.蛋白赖氨酸甲基转移酶的肿瘤抑制功能。
Exp Mol Med. 2023 Dec;55(12):2475-2497. doi: 10.1038/s12276-023-01117-7. Epub 2023 Dec 1.
3
Histone 4 lysine 20 tri-methylation: a key epigenetic regulator in chromatin structure and disease.组蛋白H4赖氨酸20三甲基化：染色质结构与疾病中的关键表观遗传调控因子

本文引用的文献

1
Plasma Histone H4 and H4K20 Trimethylation Levels Differ Between Colon Cancer and Precancerous Polyps.血浆组蛋白 H4 和 H4K20 三甲基化水平在结肠癌和癌前息肉之间存在差异。
In Vivo. 2019 Sep-Oct;33(5):1653-1658. doi: 10.21873/invivo.11651.
2
Epigenetic modulation of BRCA-1 and MGMT genes, and histones H4 and H3 are associated with breast tumors.BRCA-1 和 MGMT 基因以及组蛋白 H4 和 H3 的表观遗传调节与乳腺癌有关。
J Cell Biochem. 2019 Aug;120(8):13726-13736. doi: 10.1002/jcb.28645. Epub 2019 Apr 2.
3
miR-29a contributes to breast cancer cells epithelial-mesenchymal transition, migration, and invasion via down-regulating histone H4K20 trimethylation through directly targeting SUV420H2.
Front Genet. 2023 Aug 21;14:1243395. doi: 10.3389/fgene.2023.1243395. eCollection 2023.
4
Epigenetic Changes Associated with Osteosarcoma: A Comprehensive Review.表观遗传学改变与骨肉瘤的关系：全面综述。
Cells. 2023 Jun 9;12(12):1595. doi: 10.3390/cells12121595.
5
Protein Profiling of during Mesquite Infection.牧豆树感染期间的蛋白质谱分析
Plants (Basel). 2023 Jan 19;12(3):464. doi: 10.3390/plants12030464.
6
Lysine methyltransferase inhibitors: where we are now.赖氨酸甲基转移酶抑制剂：我们目前的进展
RSC Chem Biol. 2021 Dec 13;3(4):359-406. doi: 10.1039/d1cb00196e. eCollection 2022 Apr 6.
7
Replication Stress, Genomic Instability, and Replication Timing: A Complex Relationship.复制压力、基因组不稳定性和复制时间：复杂的关系。
Int J Mol Sci. 2021 Apr 30;22(9):4764. doi: 10.3390/ijms22094764.
miR-29a 通过直接靶向 SUV420H2 下调组蛋白 H4K20 三甲基化，从而促进乳腺癌细胞上皮-间充质转化、迁移和侵袭。
Cell Death Dis. 2019 Feb 21;10(3):176. doi: 10.1038/s41419-019-1437-0.
4
Histone H4 Methyltransferase Suv420h2 Maintains Fidelity of Osteoblast Differentiation.组蛋白H4甲基转移酶Suv420h2维持成骨细胞分化的稳定性。
J Cell Biochem. 2017 May;118(5):1262-1272. doi: 10.1002/jcb.25787. Epub 2016 Nov 30.
5
Osteosarcoma Genetics and Epigenetics: Emerging Biology and Candidate Therapies.骨肉瘤的遗传学与表观遗传学：新兴生物学与候选疗法
Crit Rev Oncog. 2015;20(3-4):173-97. doi: 10.1615/critrevoncog.2015013713.
6
Advances in emerging drugs for osteosarcoma.骨肉瘤新型药物的进展
Expert Opin Emerg Drugs. 2015 Sep;20(3):495-514. doi: 10.1517/14728214.2015.1051965. Epub 2015 Jun 1.
7
SUV420H2 suppresses breast cancer cell invasion through down regulation of the SH2 domain-containing focal adhesion protein tensin-3.SUV420H2通过下调含SH2结构域的粘着斑蛋白张力蛋白3来抑制乳腺癌细胞的侵袭。
Exp Cell Res. 2015 May 15;334(1):90-9. doi: 10.1016/j.yexcr.2015.03.010. Epub 2015 Mar 23.
8
Critical roles of non-histone protein lysine methylation in human tumorigenesis.赖氨酸甲基化在人类肿瘤发生中的关键作用。
Nat Rev Cancer. 2015 Feb;15(2):110-24. doi: 10.1038/nrc3884.
9
Histone trimethylation at H3K4, H3K9 and H4K20 correlates with patient survival and tumor recurrence in early-stage colon cancer.H3K4、H3K9和H4K20位点的组蛋白三甲基化与早期结肠癌患者的生存率和肿瘤复发相关。
BMC Cancer. 2014 Jul 22;14:531. doi: 10.1186/1471-2407-14-531.
10
Loss of histone H4K20 trimethylation predicts poor prognosis in breast cancer and is associated with invasive activity.组蛋白H4K20三甲基化缺失预示乳腺癌预后不良，并与侵袭活性相关。
Breast Cancer Res. 2014 Jun 22;16(3):R66. doi: 10.1186/bcr3681.