血浆组蛋白 H4 和 H4K20 三甲基化水平在结肠癌和癌前息肉之间存在差异。
Plasma Histone H4 and H4K20 Trimethylation Levels Differ Between Colon Cancer and Precancerous Polyps.
机构信息
Department of Basic Oncology at Oncology Institute, Istanbul University, Istanbul, Turkey.
Department of General Surgery, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey.
出版信息
In Vivo. 2019 Sep-Oct;33(5):1653-1658. doi: 10.21873/invivo.11651.
BACKGROUND/AIM: No blood-based biomarkers are available to differentiate between colonic tumors and precancerous polyps. Previously we demonstrated levels of trimethylated H4K20 (H4K20me3) to be lower in blood plasma from patients with colon cancer than those from cancer-free individuals. Herein, we added individuals with precancerous polyps for the first time in order to analyze and investigate the usefulness of plasma H4K20me3 and histone H4 to discriminate colon tumors from precancerous polyps.
MATERIALS AND METHODS
The study included a cohort of 185 individuals undergoing colonoscopy. H4K20me3 and histone H4, measured by an enzyme-linked immunosorbent assay-like assay in plasma, were analyzed according to colonoscopy findings.
RESULTS
Levels of H4K20me3 were lower in patients with colon cancer than in individuals with normal colonoscopy and those with precancerous polyps (p=0.02 and p=0.01, respectively). In contrast, highest quantities of histone H4 were measured in those with colon cancer compared to other groups (all p<0.01).
CONCLUSION
Beside H4K20me3, plasma histone H4 is a useful marker to discriminate colonic tumors from precancerous polyps and other conditions.
背景/目的：目前尚无基于血液的生物标志物可用于区分结肠肿瘤和癌前息肉。我们先前证明，结肠癌患者血浆中的三甲基化 H4K20（H4K20me3）水平低于无癌个体。在此，我们首次将患有癌前息肉的个体纳入其中，以分析和研究血浆 H4K20me3 和组蛋白 H4 区分结肠肿瘤和癌前息肉的有用性。
材料和方法
该研究纳入了 185 名接受结肠镜检查的个体。通过酶联免疫吸附试验样测定法在血浆中分析 H4K20me3 和组蛋白 H4，并根据结肠镜检查结果进行分析。
结果
与正常结肠镜检查和患有癌前息肉的个体相比，结肠癌患者的 H4K20me3 水平较低（p=0.02 和 p=0.01）。相比之下，患有结肠癌的个体中组蛋白 H4 的含量最高（均 p<0.01）。
结论
除 H4K20me3 外，血浆组蛋白 H4 是区分结肠肿瘤和癌前息肉及其他情况的有用标志物。
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