Elleder M
Acta Neuropathol. 1977 May 16;38(2):117-22. doi: 10.1007/BF00688557.
Histochemical study of so-called neuronal ceroid-lipofuscinoses (NCL) showed that the stored material is extractable in the unfixed state especially with alkalized or acidified chloroform-methanol mixtures when compared with other solvents. The extractability was strongly reduced or almost abolished by fixation with formaldehyde. Identical results were obtained with the type one storage material (see Elleder, 1977) in all late infantile cases and in a juvenile case studied, in which, contrary to the infantile form, the stored material displayed a significantly higher degree of extractability. As far as the extractability of the type two storage material is concerned insufficient data have been accumulated, but it seems that it does not differ significantly from the first one. In the control group of lipopigments ceroid was found to be much more extractable under identical conditions than matured lipofuscin which was almost entirely resistant to all extraction procedures. The significance of the results is discussed.
对所谓的神经元蜡样脂褐质沉积症（NCL）的组织化学研究表明，与其他溶剂相比，储存物质在未固定状态下尤其是用碱化或酸化的氯仿 - 甲醇混合物时可提取。用甲醛固定后，提取能力会大大降低或几乎完全丧失。在所有晚发性婴儿病例以及所研究的一例青少年病例中，一号储存物质（见埃勒德，1977年）都得到了相同的结果，其中，与婴儿型不同，储存物质显示出明显更高的提取程度。就二号储存物质的提取能力而言，积累的数据不足，但似乎与一号没有显著差异。在脂色素对照组中，发现蜡样质在相同条件下比成熟脂褐质更易提取，成熟脂褐质几乎完全抵抗所有提取程序。讨论了这些结果的意义。
