通过快速原子轰击质谱法鉴定胰岛素蛋白酶产生的胰岛素片段。
Identification by fast atom bombardment mass spectrometry of insulin fragments produced by insulin proteinase.
作者信息
Savoy L A, Jones R M, Pochon S, Davies J G, Muir A V, Offord R E, Rose K
机构信息
Département de Biochimie Médicale, Centre Médical Universitaire, Geneva, Switzerland.
出版信息
Biochem J. 1988 Jan 1;249(1):215-22. doi: 10.1042/bj2490215.
Abstract
We describe the isolation by reversed-phase h.p.l.c. of a number of products of the degradation of insulin by insulin proteinase and their direct analysis by fast atom bombardment mass spectrometry (f.a.b.-m.s.). Various semisynthetically labelled insulins were used, including [[2H2]GlyA1]insulin and [18O]LysB29]insulin. The results obtained confirm and extend the results obtained by non-mass-spectrometric methods [Davies, Muir, Rose & Offord (1988) Biochem. J. 249, 209-214, and papers cited therein]. Cleavage sites were identified between positions A13-A14, A14-A15, B9-B10, B13-B14, B24-B25 and B25-B26. The advantages and disadvantages of the application of f.a.b.-m.s. to such studies are discussed.
摘要
我们描述了通过反相高效液相色谱法分离胰岛素蛋白酶降解胰岛素产生的多种产物，并通过快原子轰击质谱法（f.a.b.-m.s.）对其进行直接分析。使用了各种半合成标记的胰岛素，包括[[2H2]甘氨酸A1]胰岛素和[18O]赖氨酸B29]胰岛素。所获得的结果证实并扩展了通过非质谱方法获得的结果[戴维斯、缪尔、罗斯和奥福德（1988年）《生物化学杂志》249卷，209 - 214页，以及其中引用的论文]。确定了在A13 - A14、A14 - A15、B9 - B10、B13 - B14、B24 - B25和B25 - B26位置之间的裂解位点。讨论了f.a.b.-m.s.应用于此类研究的优缺点。
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