Lan Wei-Qing, Liu Lin, Zhang Nan-Nan, Huang Xia, Weng Zhong-Ming, Xie Jing
Shanghai Ocean University College of Food Science and Technology, Shanghai, China.
Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center, National Experimental Teaching Demonstration Center for Food Science and Engineering, Shanghai Ocean University, Shanghai, China.
J Food Biochem. 2020 Oct;44(10):e13418. doi: 10.1111/jfbc.13418. Epub 2020 Aug 9.
To evaluate the quality changes in large yellow croaker (Pseudosciaena crocea) with ε-polylysine and rosemary extract stored on ice at 4 ± 1°C. About 0.1% ε-polylysine (PL) and 0.2% rosemary extract (RE) were individually or in combination with each other were treated with samples. Samples treated with deionized water were regarded as control check (CK) group. Physicochemical (texture profile analysis (TPA), pH, total volatile basis nitrogen (TVB-N), thiobarbituric acid (TBA)), endogenous enzyme activity (cathepsin B and D), proteolytic degradation (Trichloroacetic Acid (TCA)-soluble peptides and Sodium Salt-polyacrylamide gel electrophoresis (SDS-PAGE)), microbiological (Total viable count (TVC), Shewanella bacteria count, Pseudomonas bacteria count, Psychrophilic bacteria count) and sensory evaluation were conducted during the whole storage. As a result, PL + RE could delay the increase in pH, TVB-N, TBA value, and improve the texture attributes compared with the CK group. In addition, PL + RE could inhibit cathepsin B and D activities, protein degradation, and microbial growth effectively. Moreover, the shelf life of samples could be prolonged at least 4 days when compared with the CK group according to the quality index method (QIM) and physicochemical assay, indicating that the PL + RE treatment could maintain the quality of large yellow croaker more effectively. PRACTICAL APPLICATIONS: The preservation of fish is becoming increasingly important in aquatic products. According to the fence theory, a combination of biopreservatives with different functions could be used to maintain the freshness synergistically. Furthermore, this research indicates that the combination of ε-polylysine and rosemary extract, a promising method for the preservation of aquatic products, could slow down the deterioration of large yellow croaker and prolong its shelf life.
为评估在4±1°C冰藏条件下,添加ε-聚赖氨酸和迷迭香提取物的大黄鱼(Pseudosciaena crocea)的品质变化。分别用约0.1%的ε-聚赖氨酸(PL)、0.2%的迷迭香提取物(RE)单独处理或二者组合处理样品。用去离子水处理的样品作为对照(CK)组。在整个贮藏期间进行理化指标(质地剖面分析(TPA)、pH值、挥发性盐基氮(TVB-N)、硫代巴比妥酸(TBA))、内源酶活性(组织蛋白酶B和D)、蛋白水解降解(三氯乙酸(TCA)可溶性肽和十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE))、微生物指标(总活菌数(TVC)、希瓦氏菌数、假单胞菌数、嗜冷菌数)和感官评价。结果表明,与CK组相比,PL+RE能延缓pH值、TVB-N、TBA值的升高,并改善质地特性。此外,PL+RE能有效抑制组织蛋白酶B和D的活性、蛋白质降解以及微生物生长。而且,根据质量指标法(QIM)和理化分析,与CK组相比,样品的货架期可延长至少4天,表明PL+RE处理能更有效地保持大黄鱼的品质。实际应用:鱼类保鲜在水产品中变得越来越重要。根据栅栏理论,可使用具有不同功能的生物防腐剂组合来协同保持新鲜度。此外,本研究表明,ε-聚赖氨酸和迷迭香提取物的组合是一种很有前景的水产品保鲜方法,可减缓大黄鱼的变质并延长其货架期。