Chen Yun, Wang Bo, Liu Wenming, Xu Peng, Song Li
Clin Lab. 2020 Aug 1;66(8). doi: 10.7754/Clin.Lab.2020.191266.
The current study aims to observe the expression of hsa_circ_0141720 in the serum of patients with acute cerebral infarction (ACI) and to explore its clinical value in the diagnosis of ACI.
Eighty patients with ACI within the previous 48 hours were selected, and 30 healthy persons in the same period were selected as the control. Microarray analysis was performed to evaluate the changes of circRNA profiles, and RT-PCR was used to validate the findings. Pearson's correlation assay was performed to analyze the correlation between the level of hsa_circ_0141720 and other clinical indicators.
Microarray analysis identified eight differentially expressed cirRNAs in the serum of ACI patients. RT-PCR validated that the expression of hsa_circ_0141720 in serum of ACI patients was increased the most. Hence, we mainly focused on hsa_circ_0141720 in the following study. ROC curve analysis showed that when the cutoff value for serum hsa_circ_0141720 was 2.03, the sensitivity and specificity were 89.7% and 95.6%, respectively. Further study showed that enhanced hsa_circ_0141720 expression was positively correlated with the National Institutes of Health Stroke Scale (NIHSS) scores and infarct volume of ACI patients. Moreover, upregulation of hsa_circ_0141720 was also positively correlated with increased expression of serum interleukin 6 (IL-6) and plasma high-sensitivity C relative protein (hs-CRP) in patients with ACI.
In summary, enhanced expression of hsa_circ_0141720 in the serum of patients with ACI was related to the severity of the disease and it may be used as a new serological index for the diagnosis of ACI.
本研究旨在观察急性脑梗死（ACI）患者血清中hsa_circ_0141720的表达情况，并探讨其在ACI诊断中的临床价值。
选取发病48小时内的80例ACI患者，同期选取30例健康人作为对照。进行基因芯片分析以评估环状RNA（circRNA）谱的变化，并用逆转录-聚合酶链反应（RT-PCR）验证结果。采用Pearson相关性分析来分析hsa_circ_0141720水平与其他临床指标之间的相关性。
基因芯片分析鉴定出ACI患者血清中有8种差异表达的circRNA。RT-PCR验证发现，ACI患者血清中hsa_circ_0141720的表达增加最为明显。因此，在后续研究中我们主要关注hsa_circ_0141720。受试者工作特征（ROC）曲线分析显示，当血清hsa_circ_0141720的截断值为2.03时，灵敏度和特异度分别为89.7%和95.6%。进一步研究表明，hsa_circ_0141720表达增强与ACI患者的美国国立卫生研究院卒中量表（NIHSS）评分及梗死体积呈正相关。此外，ACI患者中hsa_circ_0141720的上调也与血清白细胞介素6（IL-6）和血浆高敏C反应蛋白（hs-CRP）表达增加呈正相关。
综上所述，ACI患者血清中hsa_circ_0141720表达增强与疾病严重程度相关，它可能作为ACI诊断的一种新的血清学指标。
