不同海拔高度的刺槐蜂蜜：总酚和黄酮类化合物、激光诱导荧光（LIF）光谱及抗癌活性
Acacia honey from different altitudes: total phenols and flavonoids, laser-induced fluorescence (LIF) spectra, and anticancer activity.
作者信息
Mohammed Moammed Elimam Ahamed, Shati Ali A, Alfaifi Mohammad Y, Elbehairi Serag Eldin I, Alshehri Mohammed Ali, Alhag Sadeq K, Suleiman Mohamed H A, Ghramh Hamed A, Ibrahim Akram, Alshehri Ali M, Al-Mosa Amal Ahmed Ali, ALaerjani Wed Mohammed Ali
机构信息
Department of Chemistry, Faculty of Science, King Khalid University, Abha, Saudi Arabia.
Unit of Bee Research and Honey Production, Faculty of Science, King Khalid University, Abha, Saudi Arabia.
出版信息
J Int Med Res. 2020 Aug;48(8):300060520943451. doi: 10.1177/0300060520943451.
OBJECTIVE
To investigate Acacia honey from different altitudes regarding total phenols and flavonoids, laser-induced fluorescence (LIF) spectra and anticancer activity against human cancer cell lines.
METHODS
Anticancer activity was investigated using sulforhodamine B cytotoxicity assays in the following human cancer cell lines: HCT116 (colon); MCF7 (breast), and HepG2 (liver). Total phenols and flavonoids were measured using spectrophotometric methods and LIF was used to differentiate between low and high-altitude honey.
RESULTS
The LIF spectra differed between low and high-altitude Acacia honey. High altitude Acacia honey was characterized by significantly lower total phenol content (81.47 ± 1.25 mg gallic acid equivalent [GAE]/100 g) and increased total flavonoids (10.63 ± 0.53 mg quercetin equivalent [QE]/100 g) versus low altitude Acacia honey (91.33 ± 0.96 mg GAE/100 g and 8.78 ± 0.23 mg QE/100 g, respectively). Low altitude Acacia honey displayed increased IC values against HCT116 and MCF7 cells (264.17 ± 10.5 and 482.65 ± 20.3 µg/ml, respectively) versus high altitude Acacia honey (117.99 ± 12.7 and 189.82 ± 15.8 µg/ml, respectively).
CONCLUSIONS
High altitude Acacia honey had significantly more effective anticancer activity against HCT116 and MCF7 cells compared with low altitude honey.
目的
研究不同海拔高度的刺槐蜂蜜中的总酚和黄酮类化合物、激光诱导荧光（LIF）光谱以及对人类癌细胞系的抗癌活性。
方法
采用磺酰罗丹明B细胞毒性试验，在以下人类癌细胞系中研究抗癌活性：HCT116（结肠）、MCF7（乳腺）和HepG2（肝脏）。使用分光光度法测量总酚和黄酮类化合物，并利用LIF区分低海拔和高海拔蜂蜜。
结果
低海拔和高海拔刺槐蜂蜜的LIF光谱不同。与低海拔刺槐蜂蜜（分别为91.33±0.96mg没食子酸当量[GAE]/100g和8.78±0.23mg槲皮素当量[QE]/100g）相比，高海拔刺槐蜂蜜的总酚含量显著降低（81.47±1.25mg GAE/100g），总黄酮含量增加（10.63±0.53mg QE/100g）。与高海拔刺槐蜂蜜（分别为117.99±12.7和189.82±15.8μg/ml）相比，低海拔刺槐蜂蜜对HCT116和MCF7细胞的IC值升高（分别为264.17±10.5和482.65±20.3μg/ml）。
结论
与低海拔蜂蜜相比，高海拔刺槐蜂蜜对HCT116和MCF7细胞具有显著更强的抗癌活性。
Zotero 插件