Università degli Studi di Palermo, Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), Via Archirafi 32, 90123 Palermo, Italy.
Università degli Studi di Palermo, Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), Via Archirafi 32, 90123 Palermo, Italy.
Int J Biol Macromol. 2020 Nov 1;162:1653-1667. doi: 10.1016/j.ijbiomac.2020.07.254. Epub 2020 Aug 7.
This research focused on the aim of tackling the urgent demand of printable biomaterials, hence we synthetized and characterized three gellan gum-graft-poly(d,l-lactide-co-glycolide) copolymers (GGm-PLGA a, b and c) which differed in the graft substitution degree. We investigated the effect of the polyester chain grafted onto hydrophilic backbone of gellan gum in terms of physicochemical properties and the ability of the system to print 3D cell laden constructs. In particular, we evaluated thermo-rheological, ionotropic crosslinking, shear thinning, swelling and stability properties of these copolymers and their derived biomaterials and findings related to the degree of functionalization. Moreover, the optimization of the 3D process parameters and the effect of different water/DPBS mixtures was investigated, demonstrating the feasibility of the system to print 3D constructs. Finally, biological tests revealed that fibroblasts and chondrocytes remained viable after printing and over a culture period of seven days into scaffolds.
本研究旨在满足对可打印生物材料的迫切需求，因此我们合成并表征了三种不同接枝取代度的结冷胶-聚（丙交酯-乙交酯）共聚物（GGm-PLGA a、b 和 c）。我们研究了聚酯链在亲水结冷胶主链上接枝对其物理化学性质和系统打印 3D 细胞负载构建体能力的影响。具体而言，我们评估了这些共聚物及其衍生生物材料的热流变、离子交联、剪切稀化、溶胀和稳定性特性，并发现了与功能化程度相关的结果。此外，我们还优化了 3D 工艺参数，并研究了不同水/DPBS 混合物的影响，证明了该系统打印 3D 构建体的可行性。最后，生物学测试表明，纤维母细胞和成软骨细胞在打印后和培养的七天内仍能在支架中存活。
